内蒙古绒山羊KAP6.2基因多态性与生长性状和绒细度性状的关联分析  

Correlation Analysis of KAP6.2 Gene Polymorphism with Growth Traits and Cashmere Fineness in Inner Mongolia Cashmere Goats

作　　者：王聪亮 张政轩 白晶晶 付琪 蓝贤勇[3] 陈生会 屈雷[1] 朱海鲸[1,5,6] WANG Congliang;ZHANG Zhengxuan;BAI Jingjing;FU Qi;LAN Xianyong;CHEN Shenghui;QU Lei;ZHU Haijing(Shaanxi Cashmere Goat Engineering&Technology Research Center,Yulin College,Yulin,Shaanxi 719000;Niujialiang Regional Animal Husbandry and Veterinary Station,Yulin,Shaanxi 719000;College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712000;Shaanxi Shenao Agricultural Biotechnology Development Co.,Ltd.,Shenmu,Shaanxi 719318;Shaanxi University-Enterprise Joint Research Center for Mutton Sheep Engineering&Technology,Shenmu,Shaanxi 719318;Shaanxi Haoli Cashmere Goat Technology DevelopmentCo.,Ltd.,Zizhou,Shaanxi 718499,China)

机构地区：[1]榆林学院陕西省绒山羊工程技术研究中心,陕西榆林719000 [2]榆林市榆阳区牛家梁区域畜牧兽医站,陕西榆林719000 [3]西北农林科技大学动物科技学院,陕西杨凌712000 [4]陕西神奥农业生物技术开发股份有限公司,陕西神木719318 [5]陕西省“四主体一联合”肉羊工程技术校企联合研究中心,陕西神木719318 [6]陕西浩丽绒山羊科技发展有限公司,陕西子洲718499

出　　处：《家畜生态学报》2024年第3期16-21,共6页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金项目(31702115);国家现代农业产业园建设绒山羊产业重点项目(2020HX02);陕西省科技统筹创新工程计划项目(2014KTDZ02-01)。

摘　　要：为探讨角蛋白关联蛋白6.2(keratin-associated protein 6.2,KAP6.2)基因遗传多态性与内蒙古绒山羊生长性状和绒细度性状的相关性,以期为内蒙古绒山羊遗传育种改良提供理论依据,本研究采集598只雌性内蒙古绒山羊耳组织,提取基因组DNA并进行PCR扩增,检测KAP6.2基因的插入/缺失(Insertion/Deletion,InDel)突变,分析突变位点与生长性状和绒细度性状的相关性。结果显示,在育成羊中,该突变位点与胸围和十字部显著相关(P<0.05),与体长、胸宽和胸深极显著相关(P<0.01);在成年羊中,该突变位点与胸宽显著相关(P<0.05),与胸围、十字部高和胸深极显著相关(P<0.01);在育成羊和成年羊全部群体中,该突变位点与体长、胸围、十字部高、胸深和胸宽极显著相关(P<0.01),与绒细度显著相关(P<0.05)。上述结果提示,KAP6.2基因外显子区24 bp InDel突变位点与内蒙古绒山羊部分生长性状显著或极显著相关,与绒细度显著相关,可作为内蒙古绒山羊部分生长性状和绒细度性状选育的遗传标记,为内蒙古绒山羊的选育提供科学依据。This study aimed to evaluate the correlation between genetic polymorphisms of keratin-associated protein 6.2 gene,growth traits and cashmere fineness of Inner Mongolia cashmere goat,in order to provide a theoretical basis for genetic breeding improvement of Inner Mongolia cashmere goats.Ear tissues of 598 female Inner Mongolia cashmere goats were collected,genomic DNA was extracted and amplified by PCR to detect KAP6.2 gene Insertion/Deletion(InDel)mutation,and the correlation between mutation sites and growth traits and cashmere fineness traits was analyzed.The results showed that in the yearling goat,the mutant was significantly correlated with heart girth and height at hip cross(P<0.05),and extremely significantly correlated with body length,chest width and chest depth(P<0.01);in the adult goat,the mutant was significantly correlated with chest width(P<0.05)and highly correlated with heart girth and height at hip cross and chest depth(P<0.01);in all goat population,the mutant was extremely significantly correlated with body length,heart girth and height at hip cross and chest depth and chest depth(P<0.01),and significantly correlated with cashmere fineness(P<0.05).The above results indicated the 24 bp InDel mutation in the exon region of KAP6.2 gene is significantly and highly significantly correlated with the partial growth traits,and significantly correlated with cashmere fineness in Inner Mongolia cashmere goat,which can be used as a genetic marker for the selection of partial growth traits and cashmere fineness traits.It provides a scientific basis for the selection and breeding of Inner Mongolia cashmere goat.

关 键 词：KAP6.2基因 绒山羊 INDEL 关联分析 

分 类 号：S827.2[农业科学—畜牧学]