IGF1/IGF-1R通过Akt/Bad通路促进肝癌进展  

IGF1/IGF-1R Promoting Hepatocellular Carcinoma Progression by Activating the Akt/Bad Pathway

作　　者：梁娇娇 唐小龙[1] LIANG Jiaojiao;TANG Xiaolong(School of Medicine,Anhui University of Science and Technology,Huainan Anhui 232007,China;Clinical Laboratory,Maternal and Child Health Hospital Laboratory,Huainan Anhui 230007,China)

机构地区：[1]安徽理工大学医学院,安徽淮南2320007 [2]淮南市妇幼保健院检验科,安徽淮南230007

出　　处：《安徽理工大学学报（自然科学版）》2023年第6期92-100,共9页Journal of Anhui University of Science and Technology:Natural Science

基　　金：国家自然科学基金资助项目(82071862,81872017)。

摘　　要：目的为了探索影响肝癌进展的新的分子机制。方法通过免疫荧光技术、免疫组织化学技术、Western blot技术检测肝癌细胞与正常肝细胞中IGF-1R的表达水平,并且通过克隆实验、JC-1实验检测IGF-1R对肝癌细胞的增殖、凋亡的影响,同时运用Western blot实验检测IGF-1R下游信号分子Akt/Bad的蛋白磷酸化水平。结果验证了IGF-1R在肝癌细胞上的表达水平明显升高,并且促进肝癌增殖、抑制凋亡。进一步研究发现IGF-1R上调Akt、Bad分子磷酸化水平影响肝癌进展。结论IGF-1R在肝癌细胞中呈明显上调表达,并且可能通过活化Akt/Bad通路促进肝癌进展。Objective To explore new molecular mechanisms affecting the progression of hepatocellular carcinoma.Method Immunofluorescence,immunohistochemistry,and Western blotting techniques were used to detect the expression level of IGF-1R in HCC cells and normal liver cells.The effects of IGF-1R on the proliferation and apoptosis of hepatocellular carcinoma cells were detected by cloning and JC-1 methods,and the protein phosphorylation level of Akt/Bad,a downstream signaling molecule of IGF-1R,was detected by Western blotting.Result It was confirmed that the expression level of IGF-1R increased significantly in hepatocellular carcinoma cells,promoting the proliferation of hepatocellular carcinoma and inhibiting cell apoptosis.Furthermore,it was found that IGF-1R affected the progression of hepatocellular carcinoma by activating the phosphorylation level Akt/Bad molecules.Conclusion The expression of IGF-1R increased significantly in liver tissue and may promote the progression of liver cancer by activating the Akt/Bad pathway.

关 键 词：IGF1/IGF-1R 肝癌 增殖与凋亡 Akt/Bad通路 分子机制 

分 类 号：R735.7[医药卫生—肿瘤]