Enzymatic self-assembly/disassembly turns “ON”/“OFF” the mimetic hydrolytic activity of histidine nanofibers  

作　　者：Xianbao Sun Manli Wang Xiaoyang Liu Wenjun Zhan Guangqiong Xu Qiaochu Jiang Hai-Dong Xu Fuqiang Wang Gaolin Liang 

机构地区：[1]State Key Laboratory of Digital Medical Engineering,School of Biological Science and Medical Engineering,Southeast University,Nanjing,210096,China [2]Analysis Center,Nanjing Medical University,Nanjing,210029,China

出　　处：《Science China Chemistry》2024年第2期517-522,共6页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China (22234002,22204019,82172097);the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX22_0245)。

摘　　要：Mimetic enzymes are devised as alternates or supplements of natural enzymes in broad fields but regulating their activities in a switchable manner remains challenging.Herein,we proposed an enzymatic self-assembly/disassembly strategy to address this issue.A peptide molecule Nap FFEYIH(YH) was rationally designed which,after self-assembling into nanofibers,lined up the histidine moieties to form active hydrolysis centers for mimicking hydrolase activity.Enzymatic dephosphorylation of Nap FFEYp IH(Yp H) by alkaline phosphatase to yield YH also turned “ON” the hydrolase activity.In turn,phosphorylation of YH by phosphokinase epidermal growth factor receptor to yield Yp H disassembled the nanofibers and thus turned the activity “OFF”.As such,the “ON”/“OFF” of the mimetic hydrolase activities could be regulated under physiological conditions through ALP/EGFR-mediated self-assembly/disassembly of histidine nanofibers.This work provides a feasible strategy for the on-demand fabrication of artificial enzymes with controllable and superior activities.

关 键 词：DISASSEMBLY hydrolytic activity mimetic enzyme phosphorylation SELF-ASSEMBLY 

分 类 号：TQ925[轻工技术与工程—发酵工程] TB383.1[一般工业技术—材料科学与工程]