代谢组学揭示茶树嫁接黄酮类物质代谢通路  

Metabonomics Reveals the Metabolic Pathway of Flavonoids in Tea Grafting

作　　者：胡志宏 周泳臣 茶凤官 何亚梅 王艳燕 高峻[2] HU Zhihong;ZHOU Yongchen;CHA Fengguan;HE Yamei;WANG Yanyan;GAO Jun(Baise Tea Industry Development Center,Baise,Guangxi 533000;Tea College,Yunnan Agricultural University,Kunming,Yunnan 650100)

机构地区：[1]百色市茶业发展中心,广西百色533000 [2]云南农业大学茶学院,云南昆明650100

出　　处：《北方园艺》2024年第4期1-7,共7页Northern Horticulture

基　　金：国家重点研发资助项目(2022YFD1601803);云南省科技计划资助项目(202004BI090055);云南省现代农业茶叶产业技术体系建设栽培研究室资助项目(2018KJTX007)。

摘　　要：以茶树品种“云桃”嫁接前后的一芽二叶为试材,采用代谢组学方法,研究了茶树嫁接过程中黄酮类物质的代谢机制,以期为深入探索茶树嫁接的黄酮代谢途径提供参考依据。结果表明:未经过嫁接的“云桃”和嫁接后的接穗“云桃”共检测到804个代谢物,其中黄酮类代谢物有213个。经过嫁接后,“云桃”中有25个黄酮类代谢物含量增加,9个含量减少。与黄酮类差异代谢物相关的富集通路主要有花青素生物合成、类黄酮生物合成、异黄酮生物合成、黄酮和黄酮醇生物合成。Taking one bud and two leaves of tea plant variety‘Yuntao’before and after grafting as experimental materials,the metabolic mechanism of flavonoids during the grafting process of tea plant was studied using metabolomics methods,in order to provide reference for further exploration of flavonoid metabolism pathways in tea plant grafting.The results showed that a total of 804 metabolites were detected in the non grafted‘Yuntao’and the grafted scion‘Yuntao’,of which 213 were flavonoid metabolites.After grafting,the content of 25 flavonoid metabolites in‘Yuntao’increased and 9 decreased.The enrichment pathways related to flavonoid differential metabolites mainly include anthocyanin biosynthesis,flavonoid biosynthesis,isoflavone biosynthesis,flavonoid and flavonol biosynthesis.

关 键 词：代谢组 茶树 嫁接 黄酮 

分 类 号：S571.1[农业科学—茶叶生产加工]