羊肚菌遗传多样性分析及菌丝显微结构观察  

Genetic Diversity Analysis and Mycelial Microstructure Observation of Morchella

作　　者：朱西强 姜娟萍 陆中华 胡传久[3] 陆娜 孙延芳[1] ZHU Xiqiang;JIANG Juanping;LU Zhonghua;HU Chuanjiu;LU Na;SUN Yanfang(College of Life Sciences and Medicine,Zhejiang Sci-Tech University,Hangzhou,Zhejiang 310018;Zhejiang Agricultural Technology Promotion Center,Hangzhou,Zhejiang 310013;Zhejiang Academy of Forestry Sciences,Hangzhou,Zhejiang 310023;Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310024)

机构地区：[1]浙江理工大学生命科学与医药学院,浙江杭州310018 [2]浙江省农业技术推广中心,浙江杭州310013 [3]浙江省林业科学院,浙江杭州310023 [4]杭州农业科学研究院,浙江杭州310024

出　　处：《北方园艺》2024年第4期104-109,共6页Northern Horticulture

基　　金：浙江省“三农九方”科技协作计划资助项目(2022SNJF044)。

摘　　要：以收集的20株羊肚菌为试材,采用ISSR分子标记法,研究其遗传多样性与亲缘关系,并用扫描电镜分析大棚种植的2个羊肚菌不同生长发育阶段菌丝体的微观结构,以期为羊肚菌种质资源开发提供参考依据。结果表明:7个ISSR引物共扩增出35个DNA片段,其中多态性条带33个,多态比率为94.3%;在遗传相似系数为0.74时,将20个羊肚菌聚类分析为6类;扫描电镜结果表明,Me19和Me20羊肚菌菌丝分枝的显微形态均由扁平状(30 d)逐渐变得浑圆(78 d),Me19的菌丝更粗壮、致密,在大棚里长势较好。The genetic diversity and genetic relationship of 20 Morchella strains were analyzed by ISSR molecular marker method,and the microstructure of the mycelium of two Morchella strains at different growth stages in the greenhouse was analyzed by scanning electron microscopy,in dorer to provide reference for the development of Morchella germplasm resources.The results showed that a total of 35 DNA fragments were amplified by 7 ISSR primers,included 33 polymorphic bands,and the polymorphism ratio was 94.3%.When the genetic similarity coefficient was 0.74,the 20 Morchella strains were divided into 6 classes by cluster analysis.The results of scanning electron microscopy showed that the morphology of mycelium branches of Morchella Me19 and Me20 gradually changed from flat(30 days)to rounded(78 days),and the mycelium of Me19 was more robust and dense,and grew better in the greenhouse.

关 键 词：羊肚菌 ISSR 遗传多样性 扫描电镜 

分 类 号：S646.19[农业科学—蔬菜学]