儿童复发性口疮与唾液微生物群落之间关联研究  

Association between recurrent stomatitis and salivary microbiome in children

作　　者：罗晓婷 刘颖萍[1] LUO Xiaoting;LIU Yingping(Department of Stomatology,Shanxi Provincial Children′s Hospital,Taiyuan,Shanxi 030013,China)

机构地区：[1]山西省儿童医院口腔科,山西太原030013

出　　处：《中国微生态学杂志》2023年第12期1407-1409,1417,共4页Chinese Journal of Microecology

摘　　要：目的研究儿童复发性口疮与唾液微生物群落之间的关联,为治疗儿童复发性口疮提供指导。方法收集到我院口腔治疗复发性口疮的90名儿童(6~12岁)。按照病情将其分为复发组(45例)和愈合组(45例),同时抽选45名健康儿童作为对照组。收集各组儿童的唾液后分别进行革兰氏染色涂片检测、实时荧光定量PCR检测和细胞因子ELISA检测。结果涂片结果显示,复发组与愈合组相比,复发组革兰阴性球菌和革兰阴性杆菌计数明显偏低(t=6.192,P=0.025;t=3.007,P=0.095),而两组革兰阳性球菌和革兰阳性杆菌计数无显著差异。实时荧光定量PCR结果显示,愈合组与对照组相比,口腔链球菌、韦荣球菌和奈瑟菌计数无显著差异,与复发组相比,愈合组口腔链球菌、韦荣球菌和奈瑟菌显著高于复发组;复发组与对照组相比,复发组口腔链球菌、韦荣球菌和奈瑟菌显著高于对照组。ELISA结果显示,复发组患儿唾液中TNF-α和IL-6的水平高于愈合组。结论患儿口腔菌群失调导致的炎症反应可能是诱发口疮反复发作的主要诱因。研究儿童复发性口疮与唾液中微生物群落之间的关联对于治疗复发性口疮具有重要意义。Objective To explore the association between recurrent mouth sores and salivary microbial communities in children.Methods Ninety children(6 to 12 years old)with recurrent mouth ulcers in our hospital for oral treatment were enrolled and divided into recurrence group(45 cases)and healing group(45 cases)according to their conditions,while 45 healthy children were selected as the control group.Saliva of the children in each group were collected for smear detection by Gram stain,real-time fluorescence quantitative PCR detection and cytokine ELISA detection,respectively.Results Compared with the healing group,the counts of G−c and G−b in the recurrence group were significantly lower(t=6.192,P=0.025;t=3.007,P=0.095),while the counts of G+c and G+b in the two groups were not significantly different.Real-time fluorescence quantitative PCR results showed that there were no significant differences in the counts of oral Streptococcus,Veillonella and Neisseria between the healing group and control group,while the counts of oral Streptococcus,Veillonella and Neisseria in the healing group were significantly higher than those in the recurrence group.The counts of oral Streptococcus,Veillonella and Neisseria in the recurrence group were significantly higher than those in the control group.ELISA results showed that the concentrations of TNF-αand IL-6 in saliva in the recurrence group were higher than those in the healing group.Conclusion The inflammatory response due to dysbiosis in oral may be the main trigger for recurrent stomatitis in children.Studying the association between recurrent stomatitis and microbial communities in saliva of children is important for the treatment of recurrent stomatitis.

关 键 词：复发性口疮 微生物 TNF-Α IL-6 

分 类 号：R788[医药卫生—口腔医学]