The construction and optimization of engineered yeast chassis for efficient biosynthesis of 8-hydroxygeraniol  

作　　者：Yu Zhang Mengdi Yuan Xinxin Wu Qiuhui Zhang Yuzhu Wang Liming Zheng Tsan-Yu Chiu Huiming Zhang Lei Lan Feng Wang Ying Liao Xuemei Gong Shirui Yan Yun Wang Yue Shen Xian Fu 

机构地区：[1]BGI Research,Shenzhen,China [2]BGI Research,Hangzhou,China [3]Guangdong Provincial Key Laboratory of Genome Read and Write,BGI Research,Shenzhen,China [4]BGI Research,Changzhou,China

出　　处：《mLife》2023年第4期438-449,共12页微生物（英文）

基　　金：supported by grants from the National Key Research and Development Program of China (No.2021YFC2100800 to Y.Z.);the Science,Technology and Innovation Commission of Shenzhen Municipality under grant (No.JCYJ20180507183534578);the Tip-top Scientific and Technical Innovative Youth Talents of Guangdong Special Support Program (2019TQ05Y876 to Y.S.)。

摘　　要：Microbial production of monoterpenoid indole alkaloids(MIAs)provides a sustainable and eco-friendly means to obtain compounds with high pharmaceutical values.However,efficient biosynthesis of MIAs in heterologous microorganisms is hindered due to low supply of key precursors such as geraniol and its derivative 8-hydroxygeraniol catalyzed by geraniol 8-hydroxylase(G8H).In this study,we developed a facile evolution platform to screen strains with improved yield of geraniol by using the SCRaMbLE system embedded in the Sc2.0 synthetic yeast and confirmed the causal role of relevant genomic targets.Through genome mining,we identified several G8H enzymes that perform much better than the commonly used CrG8H for 8-hydroxygeraniol production in vivo.We further showed that the N-terminus of these G8H enzymes plays an important role in cellular activity by swapping experiments.Finally,the combination of the engineered chassis,optimized biosynthesis pathway,and utilization of G8H led to the final strain with more than 30-fold improvement in producing 8-hydroxygeraniol compared with the starting strain.Overall,this study will provide insights into the construction and optimization of yeast cells for efficient biosynthesis of 8-hydroxygeraniol and its derivatives.

关 键 词：8-hydroxygeraniol biosynthesis G8H SCRAMBLE synthetic yeast 

分 类 号：Q93-33[生物学—微生物学]