Bioceramic scaffolds with two-step internal/external modification of copper-containing polydopamine enhance antibacterial and alveolar bone regeneration capability  

作　　者：Xiaojian JIANG Lihong LEI Weilian SUN Yingming WEI Jiayin HAN Shuaiqi ZHONG Xianyan YANG Zhongru GOU Lili CHEN 

机构地区：[1]Department of Oral Medicine,the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,310009,China [2]Bio-nanomaterials and Regenerative Medicine Research Division,Zhejiang–California International Nanosystem Institute,Zhejiang University,Hangzhou,310058,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2024年第1期65-82,共18页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the Key Research and Development Program of Zhejiang Province Foundation(No.2019C03027);the Zhejiang Provincial Natural Science Foundation of China(No.LZ22E020002);the Scientific Research Fund of Zhejiang Provincial Education Department(No.Y202148333);the Zhejiang Provincial Basic Research for Public Welfare Funds(Nos.LGF22E030002,LGF21H140001,and LTGY23H140005),China.

摘　　要：Magnesium-doped calcium silicate(CS)bioceramic scaffolds have unique advantages in mandibular defect repair;however,they lack antibacterial properties to cope with the complex oral microbiome.Herein,for the first time,the CS scaffold was functionally modified with a novel copper-containing polydopamine(PDA(Cu^(2+)))rapid deposition method,to construct internally modified(*P),externally modified(@PDA),and dually modified(*P@PDA)scaffolds.The morphology,degradation behavior,and mechanical properties of the obtained scaffolds were evaluated in vitro.The results showed that the CS*P@PDA had a unique micro-/nano-structural surface and appreciable mechanical resistance.During the prolonged immersion stage,the release of copper ions from the CS*P@PDA scaffolds was rapid in the early stage and exhibited long-term sustained release.The in vitro evaluation revealed that the release behavior of copper ions ascribed an excellent antibacterial effect to the CS*P@PDA,while the scaffolds retained good cytocompatibility with improved osteogenesis and angiogenesis effects.Finally,the PDA(Cu^(2+))-modified scaffolds showed effective early bone regeneration in a critical-size rabbit mandibular defect model.Overall,it was indicated that considerable antibacterial property along with the enhancement of alveolar bone regeneration can be imparted to the scaffold by the two-step PDA(Cu^(2+))modification,and the convenience and wide applicability of this technique make it a promising strategy to avoid bacterial infections on implants.

关 键 词：Copper-containing polydopamine MODIFICATION Antibacterial property Bone regeneration Angiogenesis Bioceramic scaffold 

分 类 号：R783.1[医药卫生—口腔医学]