矿泉水中铜绿假单胞菌检测能力的验证与分析  

Validation and Analysis of Detection Capability of Pseudomonas aeruginosa in Mineral Water

作　　者：王同智 朱文斌 张洪伟 李俊霞 周浩 WANG Tongzhi;ZHU Wenbin;ZHANG Hongwei;LI Junxia;ZHOU Hao(Chengdu Institute of Food Inspection,Chengdu 611130,China;Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation,Beijing 100029,China;Irradiation Preservation Key Laboratory of Sichuan Province,Chengdu 611130,China)

机构地区：[1]成都市食品检验研究院,四川成都611130 [2]国家市场监管重点实验室(营养与健康化学计量与应用),北京100029 [3]辐照保藏四川省重点实验室,四川成都611130

出　　处：《食品安全导刊》2024年第3期78-81,共4页China Food Safety Magazine

摘　　要：通过参加外单位组织的矿泉水中铜绿假单胞菌检测能力验证,探索减少过滤后菌落数的计数方式和范围,提高实验室应用GB 8538—2022进行检测的能力,同时验证MALDI-TOF MS的准确性。依据组织方的作业指导书对样品进行处理,采取25 mL+225 mL的方式进行稀释,根据GB 8538—2022进行检测,同时应用MALDI-TOF MS进行鉴定。样品23-V717、23-H802报告结果的评价Z值分别为-0.1、-0.9,皆为满意。此方式提高了实验员检测水平,验证了借助基质辅助激光解吸电离飞行时间质谱(Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry,MALDI-TOF MS)鉴定的准确性,缩短了检验检测时间。By participating in the validation of the detection ability of Pseudomonas aeruginosa in mineral water organized by external units,exploring the counting method and range of reducing the number of colonies afterfiltration,and improving the laboratory’s ability to apply GB 8538—2022 for detection,while verifying the accuracy of MALDI-TOF MS.Processing the samples according to the capability verification work instruction,were diluted by 25 mL+225 mL,and detecting in parallel according to GB 8538—2022,and identified by MALDI-TOF MS.The Z values of 23-V717 and 23-H802 were-0.1 and-0.9 respectively.The result is satisfactory.This capability validation improved the laboratory detection capability,verified the accuracy of MALDI-TOF MS identification,and shortened the testing time.

关 键 词：基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS) 铜绿假单胞菌 检测能力 准确性 

分 类 号：TS275.1[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]