天然产物五味子乙素对口腔癌细胞增殖、凋亡及侵袭的影响  

Schisandrin B regulates cell proliferation, apoptosis, invasion of oral cancer cells

作　　者：王春晓 汤晓飞[2] 陈中坚[1] 薛梅 路云萍 刘燕 林子琦 周晓军 沈建芬 黄优 WANG Chun-xiao;TANG Xiao-fei;CHEN Zhong-jian;XUE Mei;LU Yun-ping;LIU Yan;LIN Zi-qi;ZHOU Xiao-jun;SHEN Jian-fen;HUANG You(Suzhou Stomatological Hospital,Suzhou 215000,China)

机构地区：[1]苏州口腔医院,苏州215000 [2]首都医科大学口腔医学院

出　　处：《北京口腔医学》2024年第1期16-20,共5页Beijing Journal of Stomatology

基　　金：苏州口腔医院院级科研基金项目(SZKQYY 20B007);江苏省口腔医学会第五届征集专委会中青年科研项目(Joa-2011-4)。

摘　　要：目的 研究天然产物五味子乙素(Schisandrin B,Sch B)对口腔癌细胞增殖、凋亡和侵袭的影响。方法 用不同浓度的五味子乙素分别处理口腔癌细胞株SCC15和CAL27细胞24 h和48 h,采用CCK-8法和AnnexinV-FITC/PI双标记流式细胞术,检测五味子乙素对口腔癌细胞增殖及凋亡的影响;采用Transwell实验分别检测五味子乙素对细胞侵袭的影响,采用qRT-PCR和Western Blot检测五味子乙素对Prx1 mRNA与蛋白表达的影响。结果 CCK-8和流式细胞术检测发现五味子乙素明显抑制SCC15和CAL27细胞增殖并促进细胞凋亡,呈时间和剂量依赖性(P<0.05)。Transwell实验结果显示,五味子乙素对侵袭的抑制作用呈时间和剂量依赖性。五味子乙素对Prx1 mRNA的表达无显著影响,但对其蛋白表达有显著的抑制作用(P<0.05)。结论 五味子乙素可能通过调控Prx1蛋白的表达抑制口腔癌细胞的增殖、侵袭,促进细胞凋亡。Objective To investigate the effect of schisandrin B(Sch B)on the proliferation,apoptosis and invasion on oral cancer cells.Methods The cell viability was detected by CCK8 assay.Apoptosis was measured by Annexin V-fluorescein isothiocyanate/propidium iodide staining.Cell invasion was tested using transwell matrigel invasion assay.The mRNA and protein expression of peroxiredoxin1(Prx1)was examined by qRT-PCR and Western blot.Results Sch B significantly inhibited the proliferation of SCC15 and CAL27 cells and promoted cell apoptosis in a time-dependent and dose-dependent manner(P<0.05).The inhibitory effect of Schisandrin B on invasion was time-dependent and dose-dependent.Schisandrin B had no significant effect on the expression of Prx1 mRNA,but had a significant inhibitory effect on its protein expression(P<0.05).Conclusions Sch B may inhibit the proliferation and invasion of oral cancer cells and promote cell apoptosis by regulating the expression of Prx1 protein.

关 键 词：五味子乙素 口腔癌 Prx1 增殖 凋亡 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]