The appearance of cytoplasmic cytochrome C precedes apoptosis during Drosophila salivary gland degradation  

作　　者：Shihui Long Wenxin Cao Yongyu Qiu Ruohan Deng Jiali Liu Lidan Zhang Renke Dong Fengxin Liu Sheng Li Haigang Zhao Na Li Kang Li 

机构地区：[1]Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Institute of Insect Science and Technology,School of Life Sciences,South China Normal University,Guangzhou,China [2]Guangdong Laboratory for Lingnan Modern Agriculture,South China Agricultural University,Guangzhou,China [3]Guangmeiyuan R&D Center,Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,South China Normal University,Meizhou,Guangdong Province,China [4]School of Food Science and Engineering,South China University of Technology,Guangzhou,China [5]ChemPartner PharmaTech Co.,Ltd,Jiangmen,Guangdong Province,China

出　　处：《Insect Science》2024年第1期157-172,共16页昆虫科学（英文版）

基　　金：This study was supported by Laboratory of Lingnan Modern Agriculture Project(NT2021003 to S.Li);the National Natural Science Foundation of China(Grants No.32070491 to K.Li and 31900355 to N.Li);the National Science Foundation of Guangdong Province(Grants No.2022A1515010457 to K.Li and 2022A1515011759 to N.Li).English was edited by the Nature Publishing Group.

摘　　要：Apoptosis is an important process for organism development that functions to eliminate cell damage,maintain homeostasis,and remove obsolete tissues during morphogenesis.In mammals,apoptosis is accompanied by the release of cytochrome C(Cyt-c)from mitochondria to the cytoplasm.However,whether this process is conserved in the fruit fly,Drosophila melanogaster,remains controversial.In this study,we discovered that during the degradation of Drosophila salivary gland,the transcription of mitochondria apoptosis factors(MAPFs),Cyt-c,and death-associated APAF1-related killer(Dark)encoding genes are all upregulated antecedent to initiator and effector caspases encoding genes.The proteins Cyt-c and the active caspase 3 appear gradually in the cytoplasm during salivary gland degradation.Meanwhile,the Cyt-c protein colocates with mito-GFP,the marker indicating cytoplasmic mitochondria,and the change in mitochondrial membrane potential coincides with the appearance of Cyt-c in the cytoplasm.Moreover,impeding or promoting 20E-induced transcription factor E93 suppresses or enhances the staining of Cyt-c and the active caspase 3 in the cytoplasm of salivary gland,and accordingly decreases or increases the mitochondrial membrane potential,respectively.Our research provides evidence that cytoplasmic Cyt-c appears before apoptosis during Drosophila salivary gland degradation,shedding light on partial conserved mechanism in apoptosis between insects and mammals.

关 键 词：active caspase 3 APOPTOSIS CYT-C DROSOPHILA salivary gland 

分 类 号：Q96[生物学—昆虫学]