1株具有蛋白降解能力的雪茄优势菌株鉴定与培养条件优化  

Identification and Culture Condition Optimization of a Cigar Dominant Strain with Protein Degradation Ability

作　　者：李林林 王剑[1] 张贝贝 周亚彬 刘佳琪 黄友谊[3] 王荣浩 潘勇[1] LI Linlin;WANG Jian;ZHANG Beibei;ZHOU Yabin;LIU Jiaqi;HUANG Youyi;WANG Ronghao;PAN Yong(China Tobacco Hubei Industry LLC,Wuhan,Hubei 430040;School of Life Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070;School of Horticulture&Forestry Sciences,Huazhong Agricultural University,Wuhan,Hubei 430070,China)

机构地区：[1]湖北中烟工业有限责任公司,湖北武汉430040 [2]华中农业大学生命科学技术学院,湖北武汉430070 [3]华中农业大学园艺林学学院,湖北武汉430070

出　　处：《贵州农业科学》2024年第2期70-76,共7页Guizhou Agricultural Sciences

基　　金：湖北中烟工业有限责任公司科技项目“雪茄原料发酵和古巴雪茄风格特征功能菌落筛选”(2021JCYL3SX2B011)。

摘　　要：【目的】探明雪茄烟发酵所需优势菌株的最佳培养基配方和最优培养条件,为雪茄烟叶人工接种发酵提供菌种来源和菌株规模制备提供科学依据。【方法】从雪茄烟叶中分离出1株具有蛋白降解能力的优势菌株GL2,通过16S rDNA序列比对和生理生化特性进行鉴定,并通过单因素试验和正交试验优化培养基配方和培养条件。【结果】菌株GL2为沙福芽孢杆菌(Bacillus safensis),最佳培养基配方为牛肉膏10 g/L、酵母提取物35 g/L、MgSO_(4)0.25 g/L;最适培养条件为接种量2%(v/v)、装液量30 m L/250m L、种龄16 h。菌株GL2在最佳培养基配方和最适培养条件下培养24 h后,生物量达1.153×10^(10)CFU/m L,是优化前的4400倍。【结论】优化后培养基成分和培养条件能促进沙福芽孢杆菌(Bacillus safensis)菌株GL2的生长,有利于该菌株大规模制备与应用。【Objective】The optimal medium formula and culture conditions of the dominant strains for cigar fermentation were explored to provide the scientific basis for providing strain sources and strain scale preparation for artificial inoculation and fermentation of cigar tobacco.【Method】A dominant strain GL2 with protein degradation ability was isolated from cigar tobacco leaves.The strain was identified through 16S rDNA sequence alignment and physiological and biochemical characteristics,and the medium formula and culture conditions were optimized by single factor test and orthogonal test.【Result】The strain GL2 was identified as Bacillus safensis.The optimal medium formula included 10 g/L beef extract,35 g/L yeast extract and 0.25 g/L MgSO_(4).The optimal culture conditions were 2%inoculation amount(v/v),30 mL/250 mL liquid loading and 16 h of seed age.The biomass reached 1.153×10^(10) CFU/mL,which was 4400 times higher than that before the optimization after the strain GL2 cultured for 24 h under the optimal medium formula and optimum culture conditions.【Conclusion】The optimized medium composition and culture conditions can promote the growth of B.safensis strain GL2,which is conducive to the large-scale preparation and application of the strain.

关 键 词：雪茄 优势菌株 菌株鉴定 沙福芽孢杆菌 培养条件优化 生物量 

分 类 号：S572[农业科学—烟草工业]