黄芪甲苷对地塞米松诱导的骨质疏松模型C57BL/6小鼠RAAS系统的影响  

Cleptic Astragalus metatoside on the osteoporosis model induced by dexamethasone C57BL/6 mice regulatory RASS system effect

作　　者：柴艺汇 牛建均 田兴中 刘春艳 陈云志 吴大梅 Chai Yihui;Niu Jianjun;Tian Xingzhong;Liu Chunyan;Chen Yunzhi;Wu Damei(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China;Dejiang County Hospital of Traditional Chinese Medicine for Nationalities,Tongren 565200,Guizhou,China;Zhangjiakou Fifth Hospital,Zhangjiakou 075000,Hebei,China.)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]德江县民族中医院,贵州铜仁565200 [3]张家口市第五医院,河北张家口075000

出　　处：《贵州医药》2024年第2期180-183,190,共5页Guizhou Medical Journal

基　　金：贵州省卫生健康委科学技术基金项目(gzwkj2023-524)。

摘　　要：目的探究黄芪甲苷对骨质疏松模型小鼠维生素D系统和肾素-血管紧张素-醛固酮系统通路的影响,以期进一步阐明黄芪甲苷治疗骨质疏松的机制。方法将30只C57BL/6雄性小鼠随机分为六组:空白组、模型组、黄芪甲苷低、中、高剂量组(6、12、25 mg/kg)、维生素D组(0.7μg/kg,1,25(OH)_(2)D_(3)),每组5只。采用HE染色法观察黄芪甲苷对地塞米松诱导的骨质疏松模型C57BL/6小鼠骨组织病理学改变;采用ELISA法检测黄芪甲苷对地塞米松诱导的骨质疏松模型C57BL/6小鼠血清AKP、25(OH)D_(3)的影响;采用Western-blot检测黄芪甲苷对地塞米松诱导的骨质疏松模型C57BL/6小鼠股骨VDR、FGF23、ACE、AngⅡ蛋白表达的影响。结果与空白组比较,模型组小鼠股骨骨皮质粗糙,破骨细胞增多,伴随凹坑吸收区域的增大,骨小梁变细,数量减少,间隙增大;与模型组比较,黄芪甲苷低、中、高剂量组小鼠股骨骨小梁变细、数量减少程度均明显减轻。与空白组比较,模型组小鼠血清AKP、25(OH)D_(3)含量显著降低(P<0.01);模型组小鼠股骨VDR蛋白表达量显著降低(P<0.01),FGF23、ACE、AngⅡ蛋白表达量显著升高(P<0.01)。与模型组比较,黄芪甲苷高、中剂量组和维生素D组小鼠血清AKP、25(OH)D_(3)含量显著升高(P<0.01);黄芪甲苷高、中剂量组和维生素D组小鼠股骨VDR蛋白表达量显著升高(P<0.01),FGF23、ACE、AngⅡ蛋白表达量显著降低(P<0.01)。结论黄芪甲苷可防治地塞米松诱导的C57BL/6J小鼠骨质疏松,其机制可能通过调节FGF23、ACE、AngⅡ蛋白表达有关。Objective To search influence of Cleptic Astragalus to osteoporosis mice model vitamin D system and the renin-vascular tension-aldosterone systemic pathways,and elucidate the molecular mechanism of the Cleptic Astragalus therapy osteoporosis.Methods Thirty C57BL/6 male mice were randomly divided into 6 groups which were blank group,model group,astragalus metacoside low and medium and high dose group(6 mg/(kg.d),12 mg/(kg.d),25mg/(kg.d)],vitamin D(VD) group(0.7μg/(kg·d),1,25(OH)2D_(3)),and each group of 5 mice.Observed osteoporosis pathological changes for Cleptic Astragalus to dexamethasone induced osteoporosis model C57BL/6 mouse by HE staining method.Mice model serum influence was detected AKP,25(OH)D_(3) by ELISA,and the effect of VDR,FGF23,ACE,and AngII protein expression by western-blot(WB).Results Compared with the blank group,the model group mice had rough cortex and increased bone cells.With the increase in the absorption area of the concave pit,the bone beams become thinner,the number was reduced,and the gap increased.The small and high-dose mouse bone beams were thinner and the number was significantly reduced compared with model group.Compared with the blank group,the content of the model group mice AKP,25(OH)D_(3) was significantly reduced(P<0.01),the model group mouse femoral VDR and protein expression decreased significantly(P<0.01),FGF23,ACE,ANGI and protein expression increased significantly(P<0.01).Compared with the model group,the content of AKP,25(OH)D_(3) in the medium and high dose group and vitamin group were significantly increased(P<0.01),rat femoral VDR protein expression was increased significantly(P<0.01),FGF23,ACE,AngⅡ protein expression was significantly reduced(P<0.01).Conclusion Cleptic Astragalus can control and treat the osteoporosis by dexamethasone induced osteoporosis model C57BL/6J mice,and mechanism may be that the expression of FGF23 and ACE and AngⅡ protein are regulated by Clepltic Astragalus therapy.

关 键 词：黄芪甲苷 维生素D RAAS 骨质疏松 

分 类 号：R363[医药卫生—病理学]