我国华东与华南地区养殖鱼类迟缓爱德华氏菌分离株的多样性分析  

Diversity analysis of Edwardsiella tarda isolates from cultured fish in Eastern and Southern China

作　　者：李素一[1,2] 池洪树 陈斌[3] 张晓佩 许斌福 LI Su-yi;CHI Hong-shu;CHEN Bin;ZHANG Xiao-pei;XU Bin-fu(Institute of Biotechnology,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China;Fujian Engineering Technology Research Center of Acquaculture Disease Control and Prevention,Fuzhou 350003,China;Freshwater Fisheries Research Institute of Fujian Province,Fuzhou 350003,China;Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China)

机构地区：[1]福建省农业科学院生物技术研究所,福州350003 [2]福建省水产病害防控工程技术研究中心,福州350003 [3]福建省淡水水产研究所,福州350003 [4]福建省农业科学院畜牧兽医研究所,福州350013

出　　处：《中国人兽共患病学报》2024年第2期147-153,共7页Chinese Journal of Zoonoses

基　　金：福建省重大专项(No.2021NZ033025、No.2020NZ08003);福建省公益类科研院所专项(No.2022R1027005、No.2023R1025006)联合资助。

摘　　要：目的对17株来源于我国华东与华南地区的养殖鱼类迟缓爱德华氏菌(Edwardsiella tarda)分离株进行多样性分析。方法通过菌体血清凝集试验及16S rRNA与hsp60部分基因序列的聚类分析。结果菌体血清凝集试验结果显示,鳗源迟缓爱德华氏菌ETY的抗O血清可凝集大部分鱼源迟缓爱德华氏菌;人源迟缓爱德华氏菌标准株ATCC15947的抗O血清仅与鱼源迟缓爱德华氏菌WY37有交叉凝集;鲶鱼爱德华氏菌EIV的抗O血清与鱼源迟缓爱德华氏菌无交叉凝集。2株鱼源迟缓爱德华氏菌AL60306NA1和E29L与以上3种爱德华氏菌抗O血清不发生凝集反应。基于16S rRNA及hsp60部分基因序列构建的系统发育进化树显示,来源于不同地区的绝大部分鱼源迟缓爱德华氏菌分离株进化同源性较高,在基于16S rRNA构建的系统进化树中,山东地区分离自大菱鲆的迟缓爱德华氏菌聚为一支。3株鱼源迟缓爱德华氏菌AL60306NA1、E29L、GK4与人源迟缓爱德华氏菌ATCC15947及人源鲶鱼爱德华氏菌EIV有一定亲缘关系。结论我国华东、华南地区的鱼源迟缓爱德华氏菌分离株普遍具有相似的血清型和较高的进化同源性,并与人源的迟缓爱德华氏菌、人源的鲶鱼爱德华氏菌有明显差异。个别鱼源迟缓爱德华氏菌血清型与分子系统进化分析结果不一致,提示在防控鱼类爱德华氏菌病时,应将菌体表面抗原的免疫原性与分子聚类分析相结合。This study was aimed at analyzing the diversity of 17 isolates of Edwardsiella tarda from cultured fish originating from Eastern and Southern China.The methods included bacterial serum agglutination tests and cluster analysis of 16S rRNA and partial gene sequences of hsp 60.Bacterial serum agglutination tests revealed that the O antigen serum of E.tarda ETY(from Anguilla japonica)agglutinated most fish-derived E.tarda.In contrast,the O antigen serum of human-derived E.tarda ATCC15947 cross-agglutinated with only fish-derived E.tarda WY37.The O antigen serum of Edwardsiella ictaluri EIV did not cross-agglutinate with fish-derived E.tarda.Notably,two fish-derived E.tarda strains,AL60306NA1 and E29L,did not exhibit agglutination reactions with the O antigen serum of the three above Edwardsiella strains.Furthermore,the phylogenetic tree constructed on the basis of 16S rRNA and partial gene sequences of hsp 60 revealed that most fish-derived E.tarda isolates from different regions exhibited high homology.Notably,the E.tarda isolates obtained from turbot in Shandong province exhibited clustering within the 16S rRNA-based phylogenetic tree.Additionally,three fish-derived E.tarda strains,AL60306NA1,E29L,and GK4,displayed a genetic relationship with human-derived E.tarda ATCC15947 and human-derived E.ictaluri EIV.In conclusion,fish-derived E.tarda isolates from Eastern and Southern China generally exhibit similarities in serotypes and share high homology.Notably,they differ significantly from human-derived E.tarda and human-derived E.ictaluri.Some fish-derived E.tarda serotypes do not align with the results of molecular phylogenetic analysis,thus underscoring the importance of combining the immunogenicity of bacterial surface antigens with molecular-level cluster analysis in efforts to control edwardsiellosis in fish.

关 键 词：迟缓爱德华氏菌 血清型 16S rRNA HSP60 系统进化树 

分 类 号：R378.2[医药卫生—病原生物学] S917.1[医药卫生—基础医学]