Method for the extraction of circulating nucleic acids based on MOF reveals cell-free RNA signatures in liver cancer  

作　　者：Yuqing Sun Haixin Yu Shaoqing Han Ruoxi Ran Ying Yang Yongling Tang Yuhao Wang Wenhao Zhang Heng Tang Boqiao Fu Boshi Fu Xiaocheng Weng Song-Mei Liu Hexiang Deng Shuang Peng Xiang Zhou 

机构地区：[1]College of Chemistry and Molecular Sciences,Key Laboratory of Biomedical Polymers-Ministry of Education,Wuhan University,Wuhan 430072,China [2]Department of Digestive Surgical Oncology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China [3]Cancer Center,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China [4]Department of Clinical Laboratory,Center for Gene Diagnosis and Program of Clinical Laboratory,Zhongnan Hospital of Wuhan University,Wuhan 430071,China [5]College of Chemistry and Materials Science,Hubei Engineering University,Xiaogan 432000,China [6]Department of Pharmacology,School of Pharmacy,China Medical University,Shenyang 110122,China [7]Taikang Center for Life and Medical Sciences,Wuhan University,Wuhan 430071,China [8]Department of Hematology,Zhongnan Hospital of Wuhan University,Wuhan 430071,China

出　　处：《National Science Review》2024年第1期89-101,共13页国家科学评论（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (92153303, 21721005 and 91940000to X.Z.;81772276 and 81902141 to S.L.);the National Science Foundation for Young Scientists of China (21907078 to S.P.);Hubei Provincial Innovation Group (2019CFA018 to S.L.)。

摘　　要：Cell-free RNA(cf RNA) allows assessment of health, status, and phenotype of a variety of human organs and is a potential biomarker to non-invasively diagnose numerous diseases. Nevertheless, there is a lack of highly efficient and bias-free cf RNA isolation technologies due to the low abundance and instability of cf RNA.Here, we developed a reproducible and high-efficiency isolation technology for different types of cell-free nucleic acids(containing cf RNA and viral RNA) in serum/plasma based on the inclusion of nucleic acids by metal-organic framework(MOF) materials, which greatly improved the isolation efficiency and was able to preserve RNA integrity compared with the most widely used research kit method. Importantly, the quality of cf RNA extracted by the MOF method is about 10-fold that of the kit method, and the MOF method isolates more than three times as many different RNA types as the kit method. The whole transcriptome mapping characteristics of cf RNA in serum from patients with liver cancer was described and a cf RNA signature with six cf RNAs was identified to diagnose liver cancer with high diagnostic efficiency(area under curve = 0.905 in the independent validation cohort) using this MOF method. Thus, this new MOF isolation technique will advance the field of liquid biopsy, with the potential to diagnose liver cancer.

关 键 词：MOF circulating cell-free RNA biomarker liver cancer diagnosis 

分 类 号：R735.7[医药卫生—肿瘤]