How variable progenitor clones construct a largely invariant neocortex  

作　　者：Zhongfu Shen Jiajun Yang Qiangqiang Zhang Kuiyu Wang Xiaohui Lv Xiaolin Hu Jian Ma Song-Hai Shi 

机构地区：[1]New Cornerstone Science Laboratory,IDG/McGovern Institute for Brain Research,Tsinghua-Peking Center for Life Sciences,Beijing Frontier Research Center for Biological Structure,School of Life Sciences,Tsinghua University,Beijing 100084,China [2]Department of Computer Sciences,Tsinghua University,Beijing 100084,China [3]Chinese Institute for Brain Research,Beijing 102206,China [4]College of Life Sciences,Nankai University,Tianjin 300071,China

出　　处：《National Science Review》2024年第1期124-139,共16页国家科学评论（英文版）

基　　金：supported by the STI2030-Major Projects(2021ZD0202300);the National Natural Science Foundation of China (32021002);the Beijing Outstanding Young Scientist Program (BJJWZYJH01201910003012);the Beijing Municipal Science&Technology Commission (Z221100003422011 and Z211100003321001);the Chinese Institute for Brain Research(Beijing);the New Cornerstone Investigator Program。

摘　　要：The neocortex contains a vast collection of diverse neurons organized into distinct layers. While nearly all neocortical neurons are generated by radial glial progenitors(RGPs), it remains largely unclear how a complex yet organized neocortex is constructed reliably and robustly. Here, we show that the division behavior and neuronal output of RGPs are highly constrained with patterned variabilities to support the reliable and robust construction of the mouse neocortex. The neurogenic process of RGPs can be well-approximated by a consistent Poisson-like process unfolding over time, producing deep to superficial layer neurons progressively. The exact neuronal outputs regarding layer occupation are variable;yet, this variability is constrained systematically to support all layer formation, largely reflecting the variable intermediate progenitor generation and RGP neurogenic entry and exit timing differences. Together, these results define the fundamental features of neocortical neurogenesis with a balanced reliability and variability for the construction of the complex neocortex.

关 键 词：NEOCORTEX radial glial progenitor intermediate progenitor NEUROGENESIS clonal variability layer formation 

分 类 号：Q189[生物学—神经生物学]