Multi-region calcium imaging in freely behaving mice with ultra-compact head-mounted fluorescence microscopes  

作　　者：Feng Xue Fei Li Ke-ming Zhang Lufeng Ding Yang Wang Xingtao Zhao Fang Xu Danke Zhang Mingzhai Sun Pak-Ming Lau Qingyuan Zhu Pengcheng Zhou Guo-Qiang Bi 

机构地区：[1]Department of Precision Machinery and Precision Instruments,University of Science and Technology of China,Hefei 230026,China [2]Hefei National Laboratory for Physical Sciences at the Microscale,University of Science and Technology of China,Hefei 230026,China [3]Interdisciplinary Center for Brain Information,the Brain Cognition and Brain Disease Institute,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China [4]Faculty of Life and Health Sciences,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China [5]School of Life Sciences,University of Science and Technology of China,Hefei 230026,China [6]Institute of Semiconductors,Chinese Academy of Sciences,Beijing 100083,China [7]Department of Modern Life Sciences and Biotecnology,Xiongan Institute of Innovation,Xiongan New Area,Xiongan 071899,China [8]Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions,Shenzhen 518055,China [9]Suzhou Institute for Advanced Research,University of Science and Technology of China,Suzhou 215123,China [10]Institute of Artificial Intelligence,Hefei Comprehensive National Science Center,Hefei 230088,China

出　　处：《National Science Review》2024年第1期156-168,共13页国家科学评论（英文版）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32030200);the National Natural Science Foundation of China(NSFC)-Guangdong Joint Fund(U20A6005);the NSFC(32100903);the Key-Area Research and Development Program of Guangdong Province(2018B030331001);the Science and Technology Innovation Committee of Shenzhen Municipality(JCYJ20220818101611024)。

摘　　要：To investigate the circuit-level neural mechanisms of behavior,simultaneous imaging of neuronal activity in multiple cortical and subcortical regions is highly desired.Miniature head-mounted microscopes offer the capability of calcium imaging in freely behaving animals.However,implanting multiple microscopes on a mouse brain remains challenging due to space constraints and the cumbersome weight of the equipment.Here,we present TINIscope,a Tightly Integrated Neuronal Imaging microscope optimized for electronic and opto-mechanical design.With its compact and lightweight design of 0.43 g,TINIscope enables unprecedented simultaneous imaging of behavior-relevant activity in up to four brain regions in mice.Proof-of-concept experiments with TINIscope recorded over 1000 neurons in four hippocampal subregions and revealed concurrent activity patterns spanning across these regions.Moreover,we explored potential multi-modal experimental designs by integrating additional modules for optogenetics,electrical stimulation or local field potential recordings.Overall,TINIscope represents a timely and indispensable tool for studying the brain-wide interregional coordination that underlies unrestrained behaviors.

关 键 词：miniature microscope multiple sites calcium imaging multimodal integration HIPPOCAMPUS 

分 类 号：TP391.41[自动化与计算机技术—计算机应用技术] Q189[自动化与计算机技术—计算机科学与技术]