枸杞籽油对亚急性衰老大鼠睾丸Nrf2/ARE通路和氧化损伤的影响  

Lycium barbarum seed oil activates Nrf2/ARE pathway to reduce oxidative damage in testis of subacute aging rats

作　　者：田瑞莹 邢莎莎 虎娜 马文欣 刘畅 刘自钰 马彪 李佳阳 刘虎军 白长财[4] 陈冬梅[5] 马会明[1] TIAN Rui-ying;XING Sha-sha;HU Na;MA Wen-xin;LIU Chang;LIU Zi-yu;MA Biao;LI Jia-yang;LIU Hu-jun;BAI Chang-cai;CHEN Dong-mei;MA Hui-ming(Key Laboratory of Fertility Preservation and Maintenance,Ministry of Education,Ningxia Medical University,Yinchuan 750004,China;School of Basic Medicine,Ningxia Medical University,Yinchuan 750004,China;School of Clinical Medicine,Ningxia Medical University,Yinchuan 750004,China;School of Pharmaceutical Sciences,Ningxia Medical University,Yinchuan 750004,China;Ningxia Key Laboratory of Stem Cell and Regenerative Medicine,General Hospital of Ningxia Medical University,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学生育力保持教育部重点实验室,宁夏银川750004 [2]宁夏医科大学基础医学院,宁夏银川750004 [3]宁夏医科大学临床医学院,宁夏银川750004 [4]宁夏医科大学药学院,宁夏银川750004 [5]宁夏医科大学总医院宁夏干细胞与再生医学重点实验室,宁夏银川750004

出　　处：《中国药理学通报》2024年第3期490-498,共9页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No8186150479)。

摘　　要：目的探索枸杞籽油对亚急性衰老大鼠睾丸Nrf2/ARE通路和氧化损伤的影响。方法将60只8周龄♂SD大鼠中的50只(空白组10只)颈部皮下注射D-半乳糖125 mg·kg^(-1),持续8周建立亚急性衰老大鼠模型,β-半乳糖苷酶(β-galactosidase,β-gal)观察衰老细胞,HE观察睾丸形态,ELISA检测血清中睾酮(testosterone,T)、促卵泡激素(follicle stimulating hormone,FSH)和黄体生成素(luteinizing hormone,LH)及超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)的水平,免疫组化和Western blot检测衰老、氧化损伤和Nrf2/ARE通路相关因子表达。结果模型鉴定成功后,经枸杞籽油干预,改善了睾丸形态,下调β-gal和γH2AX表达;升高血清中SOD、GSH以及T、FSH水平及降低MDA和LH水平(P<0.05);上调睾丸组织Nrf2、GCLC、NQO1和SOD2蛋白表达水平(P<0.05)以及支持细胞中Nrf2核表达。结论枸杞籽油激活亚急性衰老大鼠睾丸Nrf2/ARE信号通路表达,降低其氧化损伤,改善其激素水平。Aim To explore the effects of Lycium berry seed oil on Nrf2/ARE pathway and oxidative damage in testis of subacute aging rats.Methods Fifty out of 60 male SD rats,aged 8 weeks,were subcutaneously injected with 125 mg·kg^(-1) D-galactosidase in the neck for 8 weeks to establish a subacute senescent rat model.The presence of senescent cells was observed usingβ-galactosidase(β-gal),while testicular morphology was examined using HE staining.Serum levels of testosterone(testosterone,T),follicle-stimulating hormone(follicle stimulating hormone,FSH),luteinizing hormone(luteinizing hormone,LH),superoxide dismutase(superoxide dismutase,SOD),glutathione(glutathione,GSH)and malondialdehyde(malondialdehyde,MDA)were measured through ELISA,and the expressions of factors related to aging,oxidative damage,and the Nrf2/ARE pathway were assessed via immunohistochemical analysis and Western blotting.Results After successfully identifying the model,the morphology of the testis was improved and the intervention of Lycium seed oil led to a down-regulation in the expression ofβ-gal andγH2AX.The serum levels of SOD,GSH,T,and FSH increased while MDA and LH decreased(P<0.05).Additionally,there was an up-regulated expression of Nrf2,GCLC,NQO1,and SOD2 proteins in testicular tissue(P<0.05)and nuclear expression of Nrf2 in sertoli cells.Conclusion Lycium barbarum seed oil may reduce oxidative damage in testes of subacute senescent rats by activating the Nrf2/ARE signaling pathway.

关 键 词：枸杞籽油 Nrf2/ARE信号通路 亚急性衰老 氧化应激 生殖激素 睾丸 

分 类 号：R-332[医药卫生] R282.71R322.64R339.38R349.1R977.12