LncRNA DLEU2对宫颈癌细胞生物学行为、miR-21和Wnt/β-catenin信号通路的影响  

Effect of lncRNA DLEU2 on biological behavior, miR-21 andWnt/β-catenin signaling pathway in cervical cancerous cells

作　　者：王文玲[1,2] 陈美华 刘旭丽[1,2] 朱开春 WANG Wenling;CHEN Meihua;LIU Xuli;ZHU Kaichun(Department of Gynecology,People′s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830000,Xinjiang,China;Xinjiang Clinical Medical Research Center for Cervical Cancer Prevention and Treatment,Urumqi 830000,Xinjiang,China)

机构地区：[1]新疆维吾尔自治区人民医院妇科,新疆乌鲁木齐市830000 [2]新疆宫颈癌防治临床医学研究中心,新疆乌鲁木齐市830000

出　　处：《广西医学》2023年第24期2990-2997,3023,共9页Guangxi Medical Journal

基　　金：新疆维吾尔自治区自然科学基金(2021D01C208);新疆维吾尔自治区人民医院院内项目(20190401)。

摘　　要：目的 探讨长链非编码RNA(lncRNA)淋巴细胞白血病缺失基因2(DLEU2)对宫颈癌细胞生物学行为的影响,并基于miR-21和Wnt/β-连环蛋白(β-catenin)信号通路探讨其作用机制。方法 将宫颈癌HeLa细胞分为对照组、DLEU2干扰组、DLEU2干扰空载组、DLEU2过表达组、DLEU2过表达空载组。除对照组外,给予其他组细胞转染相应的质粒。转染24 h后,检测各组的细胞增殖活性、细胞凋亡情况及凋亡相关蛋白表达水平、细胞周期及细胞周期相关因子的mRNA表达水平、细胞侵袭数量及上皮细胞-间充质转化(EMT)相关蛋白表达水平、miR-21表达水平、Wnt/β-catenin的蛋白及mRNA表达水平。结果 (1)与对照组相比,DLEU2过表达组的细胞增殖活性降低、细胞凋亡率增加、B细胞淋巴瘤2(Bcl-2)蛋白表达水平下调、Bcl-2相关性蛋白X和Caspase-3蛋白表达水平上调(P<0.05)。(2)与对照组相比,DLEU2过表达组的G_(1)期和S期细胞比例降低、G_(2)期细胞比例升高、细胞周期相关因子的mRNA表达水平下调(P<0.05)。(3)与对照组相比,DLEU2过表达组的细胞侵袭数减少,神经型钙黏蛋白表达水平下调,上皮型钙黏蛋白表达水平上调(P<0.05)。(4)与对照组相比,DLEU2过表达组的miR-21表达水平及Wnt3a、β-catenin的mRNA和蛋白表达水平均下调。(5)DLEU2干扰组的上述指标变化均与DLEU2过表达组相反(P<0.05),而两个空载组与对照组相比差异无统计学意义(P>0.05)。结论 DLEU2可能通过调控miR-21及抑制Wnt/β-catenin信号通路活性,从而影响宫颈癌细胞的存活、死亡、EMT和侵袭等生物学行为。Objective To explore the effect of long non-coding RNA(lncRNA)deleted in lymphocytic leukemia 2(DLEU2)on biological behavior of cervical cancerous cells,and to investigate its mechanism based on miR-21 and Wnt/β-catenin signaling pathway.Methods Cervical cancerous HeLa cells were assigned to control group,DLEU2 interfering group,DLEU2 interfering empty carrier group,DLEU2 over-expression group,or DLEU2 over-expressed empty carrier group.Except for the control group,cells of the remaining groups were transfected with corresponding plasmids.After 24 hours of transfection,cell proliferation activity,cell apoptosis and expressions of apoptosis-related proteins,cell cycle and mRNA expressions of cell cycle related factors,cell invasion number and expressions of epithelial-mesenchymal transition(EMT)related proteins,miR-21 expression,and Wnt/β-catenin protein and mRNA expressions were detected in various groups.Results(1)Compared with the control group,the DLEU2 over-expression group exhibited decreased cell proliferation activity,an increased rate of cell apoptosis,a down-regulated B cell lymphoma 2(Bcl-2)protein expression,and up-regulated Bcl-2 associated protein X and Caspase-3 protein expressions(P<0.05).(2)Compared with the control group,the DLEU2 over-expression group depicted decreased proportions of cells in phase G 1 and phase S,and an elevated proportion of cells in phase G 2,and down-regulated mRNA expressions of cell cycle related factors(P<0.05).(3)Compared with the control group,the DLEU2 over-expression group yielded less number of cell invasions,a down-regulated expression of neural cadherin,an up-regulated expression of epithelial cadherin(P<0.05).(4)Compared with the control group,the DLEU2 over-expression group interpreted a down-regulated miR-21 expression,and down-regulated mRNA and protein expressions of Wnt3a andβ-catenin(P<0.05).(5)Changes of the aforementioned indices in the DLEU2 interfering group were opposite to those in the DLEU2 over-expression group(P<0.05),whereas there was no statis

关 键 词：宫颈癌 长链非编码RNA 淋巴细胞白血病缺失基因2 细胞增殖 细胞凋亡 细胞周期 侵袭能力 上皮细胞-间充质转化 微小RNA-21 Wnt/β-连环蛋白信号通路 

分 类 号：R737.33[医药卫生—肿瘤]