氯霉素及其产物降解菌群在长期驯化过程中的微生物群落和关键基因演替模式  

The Successional Pattern of Microbial Communities and Critical Genes of Consortia Subsisting on Chloramphenicol and Its Metabolites Through Long-Term Domestication

作　　者：Jiayu Zhang Kaiyan Zhou Fangliang Guo Huaxin Lei Renxin Zhao Lin Lin Xiaoyan Li Bing Li 

机构地区：[1]State Environmental Protection Key Laboratory of Microorganism Application and Risk Control,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen 518055,China [2]Shenzhen Engineering Research Laboratory for Sludge and Food Waste Treatment and Resource Recovery,Tsinghua Shenzhen International Graduate School,Tsinghua University,Shenzhen 518055,China [3]School of Environment,Tsinghua University,Beijing 100084,China [4]Research Center for Eco-Environmental Engineering,Dongguan University of Technology,Dongguan 523808,China

出　　处：《Engineering》2023年第12期59-69,共11页工程（英文）

基　　金：funded by National Key Research and Development Program of China(2022YFE0103200);National Natural Science Foundation of China(22176107 and 22206107);Guangdong Basic and Applied Basic Research Foundation(2019B151502034 and 2021A1515110772);China Postdoctoral Science Foundation(2021M691772)。

摘　　要：As a widespread emerging contaminant,chloramphenicol(CAP)adversely impacts ecological communities in the water environment.Biological treatment is widely used for aquatic pollutant removal,and the performance of functional microbes determines its outcome.Herein,a consortium with a powerful CAPdegrading capacity was domesticated from activated sludge.As the common degradation products of CAP,4-nitrobenzoic acid(PNB)and 2,2-dichloroacetic acid(DCA)were also used as the sole substrates for long-term domestication.The successional pattern of the microbial community and critical functional genes through the 2.5-year domestication was revealed by metagenomic analysis.Sphingomonas,Caballeronia,and Cupriavidus became the most dominant populations in the CAP-,PNB-,and DCAdegrading consortia,respectively,and they were crucial degraders of PNB and DCA.Their collaboration contributed to the high mineralization rate of CAP.PNB was transformed into protocatechuic acid(PCA)and then mineralized through meta-cleavage and ortho-cleavage pathways.Crucial functional genes involved in CAP,PNB,and DCA metabolism,including CAP acetyltransferase,CAP oxidoreductase,haloacid dehalogenases,and protocatechuate dioxygenases,were significantly enriched in consortia.p H and carbon source had significant impacts on CAP biodegradation efficiency.The domesticated consortia and isolated strains are necessary microbial resources to enhance the bioremediation of CAP-,PNB-,or DCA-polluted environments.

关 键 词：Antibiotic BIODEGRADATION Metabolism Microbial community SUCCESSION METAGENOME 

分 类 号：Q938[生物学—微生物学]