血清miRNA-24和miRNA-509在肝细胞癌患者血清中的表达及与预后的关系  

The expression of serum miRNA-24 and miRNA-509 in patients with hepatocellular carcinoma and their correlation with prognosis

作　　者：庞武[1] 陈莉江 朱宇[1] 吴涯昆 张伟 PANG Wu;CHEN Lijiang;ZHU Yu;WU Yakun;ZHANG Wei(Department of hepatobiliary surgery,Suining Central Hospital,Suining 621000,Sichuan province,China;Department of Hepatobiliary Surgery,Jianyang People′s Hospital,Sichuan province)

机构地区：[1]四川省遂宁市中心医院肝胆外科,遂宁621000 [2]四川省简阳市人民医院肝胆外科

出　　处：《实用肿瘤学杂志》2023年第5期411-415,共5页Practical Oncology Journal

基　　金：成都市卫生健康委员会基金(编号:2022631)。

摘　　要：目的 探讨肝细胞癌(HCC)患者血清miRNA-24(miR-24)和miRNA-509(miR-509)的表达及临床意义。方法 选取2019年1月—2020年10月就诊于四川省遂宁市中心医院的HCC患者94例(HCC组),另选择同期体检中心健康体检者90例(对照组),采用实时定量聚合酶链反应(qRT-PCR)检测人肝癌细胞系及两组入组对象血清中miR-24、miR-509表达水平,探讨miR-24、miR-509水平与HCC临床病理特征及预后的相关性。结果 HCC组患者血清中miR-24表达量明显高于对照组(3.19±0.29vs. 0.66±0.20)(t=-68.601,P<0.01),miR-509表达量明显低于对照组(0.74±0.27vs. 1.24±0.28)(t=12.331,P<0.01)。血清中miR-24表达量与甲胎蛋白(AFP)水平、是否转移、TNM分期呈正相关(r=0.821、0.510、0.762,P<0.01),与分化程度呈负相关(r=-0.771,P<0.01)。血清中miR-509表达量同AFP水平、是否转移、TNM分期呈负相关(r=-0.820、-0.506、-0.766,P<0.01),与分化程度呈负相关(r=-0.775,P<0.01)。结论 HCC患者血清miR-24表达水平上调,而血清miR-509表达下调,同时二者均与HCC转移、恶性程度存在密切关联。临床检测患者血清中miR-24和miR-509水平有助于HCC的诊断及病情评估。Objective The objective of this study was to investigate the expression and clinical significance of serum miRNA-24(miR-24)and miRNA-509(miR-509)in patients with hepatocellular carcinoma(HCC).Methods Ninety-four HCC patients(HCC group)who visited Suining Central Hospital in Sichuan province from January 2019 to October 2020 were selected,and 90 healthy subjects(control group)who underwent the physical examination center at the same time were selected.The expression of miR-24 and miR-509 in human liver cancer cell lines and the serum of participants in the two groups was detected by real-time quantitative polymerase chain reaction(qRT-PCR).The correlation between miR-24 and miR-509 levels and the clinicopathological characteristics and prognosis of HCC was analyzed.Results The expression of miR-24 in the serum of HCC patients was significantly higher than that of the control group(3.19±0.29 vs.0.66±0.20),(t=-68.601,P<0.01),and the expression of miR-509 was significantly lower than that of the control group(0.74±0.27 vs.1.24±0.28),(t=12.331,P<0.01).The expression of miR-24 in serum was positively correlated with alpha fetoprotein(AFP)level,metastasis,and TNM stage(r=0.821,0.510,0.762,P<0.01),and negatively correlated with the degree of differentiation(r=-0.771,P<0.01).The expression of miR-509 in serum was negatively correlated with AFP level,metastasis and TNM stage(r=-0.820,-0.506,-0.766,P<0.01),and negatively correlated with the degree of differentiation(r=0.775,P<0.01).Conclusion The expression of serum mir-24 is up-regulated in HCC patients,while the expression of serum mir-509 is down-regulated.Both of them are closely related to HCC metastasis and malignancy.Clinical testing of serum miR-24 and miR-509 levels in patients can help diagnose and evaluate the condition of HCC.

关 键 词：miR-24 miR-509 肝细胞癌 肝癌转移 

分 类 号：R735.7[医药卫生—肿瘤]