柴胡加龙骨牡蛎汤对肝郁大鼠肝脏脂质代谢及SREBP-1c/FAS信号通路的影响  

Effects of Bupleurum plus Dragon’s Bone and Oyster Decoction on hepatic lipid metabolism andSREBP-1c/FAS signaling pathway in rats with liver depression syndrome

作　　者：洪捷 蔡萧君 HONG Jie;CAI Xiaojun(Heilongjiang Academy of Chinese Medicine,Harbin 150000,Heilongjiang,China)

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150000

出　　处：《现代中西医结合杂志》2024年第1期28-36,共9页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：黑龙江省中医药科研项目(ZHY2020-054)。

摘　　要：目的 探讨柴胡加龙骨牡蛎汤对肝郁大鼠肝脏脂质代谢及固醇调节元件结合蛋白-1c/脂肪酸合酶(SREBP-1c/FAS)信号通路的影响。方法 将50只雄性SPF级SD大鼠随机分为空白组、模型组、氟西汀组、柴胡加龙骨牡蛎低剂量组、柴胡加龙骨牡蛎高剂量组,每组10只。空白组正常喂养,其余组大鼠采用慢性温和不可预知应激(CUMS)方法建立肝郁模型。建模成功后,氟西汀组每天给予盐酸氟西汀0.003 g/kg灌胃,柴胡加龙骨牡蛎低、高剂量组分别每天给予柴胡加龙骨牡蛎汤1.625 g/kg和3.25 g/kg灌胃,空白组和模型组给予等体积的生理盐水灌胃,均1次/d,连续灌胃4周。分别于造模前、成模后及灌胃结束后对大鼠进行称重,进行行为学实验(糖水偏嗜实验、旷场实验、悬尾实验);灌胃结束后处死大鼠,HE染色、油红O染色观察肝脏病理变化,取血检测血脂指标[总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)]水平,采用Western blot法检测肝脏组织中SREBP-1c和FAS蛋白表达情况,采用RT-PCR法检测肝脏组织中SREBP-1c和FAS mRNA表达情况。结果 与空白组比较,灌胃结束后模型组大鼠体重、糖水偏嗜率、水平运动得分、垂直运动得分均明显降低(P均<0.05),悬尾不动时间均明显延长(P<0.05),出现肝脏增大、脂滴空泡化及肝细胞脂肪堆积,血清TG、TC、LDL-C水平均明显升高(P均<0.05),血清HDL-C水平明显降低(P<0.05),肝脏组织中SREBP-1c和FAS蛋白及mRNA相对表达量均明显升高(P均<0.05)。与模型组比较,各给药组大鼠体重、糖水偏嗜率、水平运动得分、垂直运动得分均明显增加(P均<0.05),悬尾不动时间均明显缩短(P均<0.05),肝脏病理改变明显减轻,血清TG、TC、LDL-C水平均明显降低(P均<0.05),血清HDL-C水平均明显升高(P均<0.05),肝脏组织中SREBP-1c和FAS蛋白及mRNA相对表达量均明显降低(P均<0.05)。结论 柴胡加�Objective It is to investigate the effects of Bupleurum plus Dragon’s Bone and Oyster Decoction(BDBOD)on hepatic lipid metabolism and sterol regulatory element binding protein-1c/fatty acid synthase(SREBP-1c/FAS)signaling pathway in rats with liver depression syndrome.Methods Fifty male SPF-grade SD rats were randomly divided into blank group,model group,fluoxetine group,BDBOD low-dose group and BDBOD high-dose group,with 10 rats in each group.The blank group was fed normally,and the rats in the other groups were given chronic unpredictable mild stress(CUMS)to establish models of liver depression syndrome.After successful modeling,the fluoxetine group was given fluoxetine hydrochloride 0.003 g/kg by gavage,the low and high dose groups of BDBOD were given BDBOD 1.625 g/kg and 3.25 g/kg by gavage,respectively,and the blank group and model group were given equal volumes of normal saline by gavage,all once daily,continuously treated for 4 weeks.The rats were weighed before modeling,after modeling and at the end of gavage,and were tested by behavioral experiments(sugar-water bias test,open field test,tail suspension test);these rats were executed at the end of gavage,and pathological changes of liver were observed by HE staining and oil red O staining,their blood was taken to determine the levels of lipid indices[total cholesterol(TC),triacylglycerol(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C)],and the expressions of SREBP-1c and FAS protein in liver tissues were detected by Western blot method,the expressions of SREBP-1c and FAS mRNA in liver tissues were detected by RT-PCR.Results Compared with the blank group,the body weight,sugar-water bias rate,horizontal movement score,vertical movement score of the rats in the model group were significantly decreased at the end of gavage(all P<0.05),the tail suspension immobility time was significantly increased(P<0.05),with liver enlargement,vacuolization of lipid droplets and hepatocyte fat accumulation,the serum levels of TG,T

关 键 词：肝郁 柴胡加龙骨牡蛎汤 脂质代谢 固醇调节元件结合蛋白-1C 脂肪酸合酶 

分 类 号：R-332[医药卫生]