慢性牙周炎患者血清RORA的表达水平及其与Th17/Treg细胞平衡的相关性  

The expression level of serum RORA in patients with chronic periodontitis and its correlation with the balance of Th17/Treg cells

作　　者：赵雅静 刘淑英 陈晓川 孙天语 ZHAO Yajing;LIU Shuying;CHEN Xiaochuan;SUN Tianyu(Department of Periodontology,Stomatological Hospital of Southern Medical University,Guangzhou 510200,China)

机构地区：[1]南方医科大学口腔医院牙周病科,广东广州510200

出　　处：《西部医学》2024年第3期376-381,共6页Medical Journal of West China

基　　金：广东省基础与应用基础研究基金项目(2020A1515110852)。

摘　　要：目的 探讨慢性牙周炎(CP)患者血清维甲酸受体相关孤儿受体A(RORA)的表达水平及其与辅助性T细胞17(Th17)/调节性T细胞(Treg)平衡的相关性。方法 选取2019年1月—2021年12月于本院进行检查并接受治疗的90例CP患者作为观察组,并选择同期在本院检查的牙周健康志愿者100例作为对照组,比较两组的年龄、性别、体质指数(BMI)及合并高血压、冠心病、糖尿病等一般临床资料;采用实时荧光定量PCR(qRT-PCR)检测两组血清中RORA的相对表达水平;采用流式细胞术检测两组Th17、Treg细胞水平;采用酶联免疫吸附(ELISA)法检测两组血清中转化生长因子-β(TGF-β)、白细胞介素-10(IL-10)、白细胞介素-17(IL-17)及白细胞介素-23(IL-23)的水平;采用Pearson法分析CP患者血清RORA与TGF-β、IL-10、IL-23、IL-23及Th17/Treg细胞比例之间的相关性;Logistic回归分析影响CP发生的因素。结果 两组年龄、性别、BMI及合并高血压、冠心病、糖尿病等指标比较,差异均无统计学意义(P>0.05)。观察组患者血清中Th17、IL-17、IL-23水平高于对照组,RORA、Treg、TGF-β、IL-10水平低于对照组,且Th17/Treg细胞比例高于对照组(P<0.05)。Pearson法分析结果显示,CP患者血清中RORA与TGF-β、IL-10呈正相关(P<0.05),与IL-17、IL-23及Th17/Treg细胞比例呈负相关(P<0.05)。Logistic回归分析结果显示,RORA、TGF-β、IL-10、IL-17、IL-23及Th17/Treg是CP发生的影响因素(P<0.05)。结论 CP患者血清中RORA低表达,Th17/Treg细胞比例失衡,RORA的表达与Th17/Treg细胞比例之间呈负相关,RORA可能通过调节Th17/Treg细胞比例影响CP的发生。Objective To study the expression level of retinoic acid receptor related orphan receptor A(RORA) in serum of patients with chronic periodontitis(CP) and its correlation with the balance of helper T cell 17(Th17)/regulatory T cell(Treg).Methods Ninety CP patients who were examined and treated in our hospital from January 2019 to December 2021 were set as the research group,and 100 healthy periodontal volunteers who were examined in our hospital during the same period were selected as the control group.The general clinical data such as age,gender,body mass index,hypertension,coronary heart disease,and diabetes were compared between the two groups;the relative expression level of RORA in the serum of the two groups was detected by real-time fluorescence quantitative PCR;flow cytometry was used to detect the levels of Th17 and Treg cells in the two groups;enzyme-linked immunosorbent assay was used to detect the serum levels of transforming growth factor-β(TGF-β),interleukin-10(IL-10),interleukin-17(IL-17),and interleukin-23( IL-23);Pearson′s method was used to analyze the correlation between serum RORA and TGF-β,IL-10,IL-23,IL-23 and Th17/Treg cell ratio in CP patients;Logistic regression was performed to analyze the factors affecting the occurrence of CP.Results There was no statistical significance in age,gender,body mass index,hypertension,coronary heart disease,diabetes and other indicators between the study group and the control group(P>0.05).The levels of serum Th17,IL-17 and IL-23 in the study group were higher than those in the control group,the levels of RORA,Treg,TGF-β and IL-10 were lower than those in the control group,and the ratio of Th17/Treg cells was higher than that in the control group(P<0.05).The results of Pearson′s method showed that serum RORA in CP patients was positively correlated with TGF-β and IL-10(P<0.05),and negatively correlated with IL-17,IL-23 and Th17/Treg cell ratio(P<0.05).Logistic regression analysis showed that RORA,TGF-β,IL-10,IL-17,IL-23 and Th17/Treg were the influ

关 键 词：慢性牙周炎 维甲酸受体相关孤儿受体A 辅助性T细胞17 调节性T细胞 相关性 

分 类 号：R781.42[医药卫生—口腔医学]