AP2M1抑制弥漫性大B细胞淋巴瘤细胞增殖和侵袭  

AP2M1 inhibits proliferation and invasion of diffuse large B-cell lymphoma cells

作　　者：李瑗春 严学倩 范丹 及月茹 肖方 高静 LI Yuanchun;YAN Xueqian;FAN Dan;JI Yueru;XIAO Fang;GAO Jing(Department of Hematology,the Second Affliated Hospital of Air Force Medical University,Xi′an 710038,China)

机构地区：[1]空军军医大学第二附属医院血液内科,陕西西安710001

出　　处：《基础医学与临床》2024年第3期308-316,共9页Basic and Clinical Medicine

基　　金：陕西省自然科学基础研究计划(2020JQ-460)。

摘　　要：目的探究接头相关蛋白质复合体2亚基μ1(AP2M1)对弥漫性大B细胞淋巴瘤(DLBCL)细胞增殖和侵袭的调控作用。方法将人弥漫性大B细胞淋巴瘤细胞系OCI-LY8分为对照组、NC-LV组、AP2M1-LV组。用Lipofectamine 2000进行细胞转染。四甲基偶氮唑盐(MTT)法检测细胞增殖,流式细胞仪检测细胞凋亡,Transwell小室法检测细胞迁移和侵袭。Western blot检测AP2M1、表皮生长因子受体(EGFR)、p-磷脂酰肌醇3激酶(PI3K)和p-蛋白质激酶B(AKT)蛋白表达。结果与对照组相比,AP2M1-shRNA组细胞中AP2M1的mRNA和蛋白相对表达量均降低(P<0.05),相对细胞活力升高(P<0.05),细胞凋亡率降低(P<0.05),迁移和侵袭细胞数量均升高(P<0.05);EGFR的蛋白相对表达量及PI3K和AKT的磷酸化水平均升高(P<0.05)。与对照组相比,AP2M1-LV组细胞中AP2M1的mRNA和蛋白相对表达量均升高(P<0.05),相对细胞活力降低(P<0.05),细胞凋亡率升高(P<0.05),迁移和侵袭细胞数量均降低(P<0.05),EGFR的蛋白相对表达量及PI3K和AKT的磷酸化水平均降低(P<0.05)。结论AP2M1的过表达部分通过抑制EGFR/PI3K/AKT信号通路来抑制DLBCL细胞的增殖和侵袭。Objective To evaluate the regulatory effect of the adaptor related protein complex 2 subunitμ1(AP2M1)on proliferation and invasion of diffuse large B-cell lymphoma(DLBCL).Methods Human diffuse large B-cell lymphoma cell line OCI-LY8 was aliquoted into control group,NC-shRNA group,AP2M1-shRNA group,NC-LV group,and AP2M1-LV group.Lipofectamine 2000 was used for cell transfection.Cell proliferation was detected by tetramethylazolium salt(MTT)method,apoptosis was detected by flow cytometry and cell migration and invasion were detected by Transwell assay.The protein expression of AP2M1,epidermal growth factor receptor(EGFR),p-phosphatidylinositol 3 kinase(PI3K),PI3K,p-protein kinase B(Akt)and AKT was detected by Western blot.Results Compared with control group,the relative expression of AP2M1 mRNA and protein in the AP2M1-shRNA group was decreased(P<0.05).The relative cell viability was increased(P<0.05).The cell apoptosis rate was decreased(P<0.05).The counting number of migrating and invading cells was increased(P<0.05).The relative expression level of EGFR protein and the phosphorylation level of PI3K and AKT were increased(P<0.05).Compared with Control group,the expression of AP2M1 mRNA and protein relative expression level in AP2M1-LV group was increased(P<0.05).The relative cell viability was decreased(P<0.05).The cell apoptosis rate was increased(P<0.05).The number of migrating and invading cells was decreased(P<0.05).The relative expression level of EGFR protein and the phosphorylation level of PI3K and AKT were all decreased(P<0.05).Conclusions The over-expression of AP2M1 partially inhibits the proliferation and invasion of DLBCL cells by inhibiting the EGFR/PI3K/AKT signaling pathway.

关 键 词：弥漫性大B细胞淋巴瘤 接头相关蛋白质复合体2亚基μ1(AP2M1) 表皮生长因子受体 磷脂酰肌醇3激酶 蛋白质激酶B 

分 类 号：R733.4[医药卫生—肿瘤]