Preliminary exploration of animal models of congenital choledochal cysts  

作　　者：Shu-Hao Zhang Yue-Bin Zhang Duo-Te Cai Tao Pan Ken Chen Yi Jin Wen-Juan Luo Zong-Wei Huang Qing-Jiang Chen Zhi-Gang Gao 

机构地区：[1]Department of General Surgery,Children’s Hospital,Zhejiang University School of Medicine,Hangzhou 310000,Zhejiang Province,China

出　　处：《World Journal of Gastroenterology》2024年第10期1420-1430,共11页世界胃肠病学杂志（英文版）

基　　金：the Key R&D Program of Zhejiang,No.2023C03029;Health Science and Technology Plan of Zhejiang Province,No.2022RC201;Zhejiang Provincial Natural Science Foundation Project,No.LY20H030007.

摘　　要：BACKGROUND Various animal models have been used to explore the pathogenesis of choledochal cysts(CCs),but with little convincing results.Current surgical techniques can achieve satisfactory outcomes for treatment of CCs.Consequently,recent studies have focused more on clinical issues rather than basic research.Therefore,we need appropriate animal models to further basic research.AIM To establish an appropriate animal model that may contribute to the investigation of the pathogenesis of CCs.METHODS Eighty-four specific pathogen-free female Sprague-Dawley rats were randomly allocated to a surgical group,sham surgical group,or control group.A rat model of CC was established by partial ligation of the bile duct.The reliability of the model was confirmed by measurements of serum biochemical indices,morpho-logy of common bile ducts of the rats as well as molecular biology experiments in rat and human tissues.RESULTS Dilation classified as mild(diameter,≥1 mm to<3 mm),moderate(≥3 mm to<10 mm),and severe(≥10 mm)was observed in 17,17,and 2 rats in the surgical group,respectively,while no dilation was observed in the control and sham surgical groups.Serum levels of alanine aminotransferase,aspartate aminotrans-ferase,total bilirubin,direct bilirubin,and total bile acids were significantly elevated in the surgical group as compared to the control group 7 d after surgery,while direct bilirubin,total bilirubin,and gamma-glutamyltransferase were further increased 14 d after surgery.Most of the biochemical indices gradually decreased to normal ranges 28 d after surgery.The protein expression trend of signal transducer and activator of transcription 3 in rat model was consistent with the human CC tissues.CONCLUSION The model of partial ligation of the bile duct of juvenile rats could morphologically simulate the cystic or fusiform CC,which may contribute to investigating the pathogenesis of CC.

关 键 词：Choledochal cyst Animal model Partial ligation Cystic and fusiform dilation Juvenile rats 

分 类 号：R735.9[医药卫生—肿瘤] R-332[医药卫生—临床医学]