High quality repair of osteochondral defects in rats using the extracellular matrix of antler stem cells  

作　　者：Yu-Su Wang Wen-Hui Chu Jing-Jie Zhai Wen-Ying Wang Zhong-Mei He Quan-Min Zhao Chun-Yi Li 

机构地区：[1]Institute of Antler Science and Product Technology,Changchun Sci-Tech University,Changchun 130000,Jilin Province,China [2]College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,Jilin Province,China [3]School of Life Science,Taizhou University,Taizhou 318000,Zhejiang Province,China [4]Department of Oral Implantology,Hospital of Stomatology,Jilin University,Changchun 130000,Jilin Province,China

出　　处：《World Journal of Stem Cells》2024年第2期176-190,共15页世界干细胞杂志（英文版）（电子版）

基　　金：National Natural Science Foundation of China,No.U20A20403;This study was conducted in accordance with the Animal Ethics Committee of the Institute of Antler Science and Product Technology,Changchun Sci-Tech University(AEC No:CKARI202309).

摘　　要：BACKGROUND Cartilage defects are some of the most common causes of arthritis.Cartilage lesions caused by inflammation,trauma or degenerative disease normally result in osteochondral defects.Previous studies have shown that decellularized extracellular matrix(ECM)derived from autologous,allogenic,or xenogeneic mesenchymal stromal cells(MSCs)can effectively restore osteochondral integrity.AIM To determine whether the decellularized ECM of antler reserve mesenchymal cells(RMCs),a xenogeneic material from antler stem cells,is superior to the currently available treatments for osteochondral defects.METHODS We isolated the RMCs from a 60-d-old sika deer antler and cultured them in vitro to 70%confluence;50 mg/mL L-ascorbic acid was then added to the medium to stimulate ECM deposition.Decellularized sheets of adipocyte-derived MSCs(aMSCs)and antlerogenic periosteal cells(another type of antler stem cells)were used as the controls.Three weeks after ascorbic acid stimulation,the ECM sheets were harvested and applied to the osteochondral defects in rat knee joints.RESULTS The defects were successfully repaired by applying the ECM-sheets.The highest quality of repair was achieved in the RMC-ECM group both in vitro(including cell attachment and proliferation),and in vivo(including the simultaneous regeneration of well-vascularized subchondral bone and avascular articular hyaline cartilage integrated with surrounding native tissues).Notably,the antler-stem-cell-derived ECM(xenogeneic)performed better than the aMSC-ECM(allogenic),while the ECM of the active antler stem cells was superior to that of the quiescent antler stem cells.CONCLUSION Decellularized xenogeneic ECM derived from the antler stem cell,particularly the active form(RMC-ECM),can achieve high quality repair/reconstruction of osteochondral defects,suggesting that selection of decellularized ECM for such repair should be focused more on bioactivity rather than kinship.

关 键 词：Osteochondral defect repair Mesenchymal stem cells Extracellular matrix DECELLULARIZATION Antler stem cells Reserve mesenchymal cells Xenogeneic 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]