黄花败酱HPLC多组分定量控制及PCA、OPLS-DA联合GRA法综合质量评价  

Comprehensive quality evaluation of Patrina scabiosaefolia Fisch based on HPLC multi-component quantitative control and PCA,OPLS-DA combined with gray correlation analysis

作　　者：卢立欣 郑磊 李志明 宋超 Li-Xin LU;Lei ZHENG;Zhi-Ming LI;Chao SONG(Department of Pharmacy,Shandong Provincial Third Hospital,Jinan 250000,China;School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东省立第三医院药学部,济南250000 [2]山东中医药大学药学院,济南250355

出　　处：《中国药师》2023年第12期510-518,共9页China Pharmacist

基　　金：山东省中医药科技面上项目(2021M199)。

摘　　要：目的建立HPLC法同时测定黄花败酱中常春藤皂苷元、齐墩果酸、熊果酸、黄花败酱皂苷C、原儿茶酸、绿原酸、咖啡酸、秦皮乙素、东莨菪内酯、芦丁、槲皮素和山奈酚的含量,探索主成分分析(PCA)、正交偏最小二乘法-判别分析(OPLSDA)及灰色关联度分析(GRA)模型在黄花败酱质量评价中的应用。方法采用HPLC法,色谱柱为Waters Atlantis T3 C_(18)柱(250 mm×4.6 mm,5μm),以乙腈-0.1%磷酸为流动相,梯度洗脱;检测波长为210,260,360 nm;通过对多指标成分含量结果进行PCA、OPLS-DA和GRA分析,综合评价黄花败酱的整体质量。结果外标法方法学验证结果均符合要求;12种成分在各自范围内线性关系良好(r>0.9990),平均加样回收率在96.82%~100.16%之间(RSD<2.0%,n=9)。PCA、OPLS-DA结果显示齐墩果酸、熊果酸、绿原酸和槲皮素是影响黄花败酱产品质量的主要潜在标志物。GRA结果显示相对关联度在0.3806~0.5714之间,黄花败酱存在一定批间差异。结论HPLC法同时测定黄花败酱质量中12个成分,方法简便实用。PCA、OPLS-DA和GRA可用于评价黄花败酱的整体质量。Objective To establish a HPLC method for the simultaneous determination of hederagenin,oleanolic acid,ursolic acid,scabioside C,protocatechuic acid,chlorogenic acid,caffeic acid,aesculetin,scopoletin,rutoside,quercetin,kaempferol in Patrina scabiosaefolia Fisch,and to explore the application of principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA)and grey relational analysis(GRA)in the quality evaluation of Patrina scabiosaefolia Fisch.Methods The HPLC method was used with the Waters Atlantis T3 C_(18) column(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-0.1%phosphate acid solution with gradient elution.The detection wavelengths were 210,260 and 360 nm.The overall quality of Patrina scabiosaefolia Fisch was comprehensively evaluated by PCA,OPLS-DA and GRA analysis of the results of multi-component content.Results The results of methodological verification of external standard method met the requirements.The 12 components had good linear relationships within their respective ranges(r>0.9990),and the average recovery rate were 96.82%-100.16%(RSD<2.0%,n=9).PCA and OPLS-DA results showed that oleanolic acid,ursolic acid,chlorogenic acid and quercetin were the main potential markers affecting the quality of Patrina scabiosaefolia Fisch.The relative correlation degree of GRA were 0.3806-0.5714,and there was a certain difference between batches of Patrina scabiosaefolia Fisch.Conclusion The HPLC method can simultaneously determine the 12 components in Patrina scabiosaefolia Fisch,which is simple and practical,and PCA,OPLS-DA and GRA can be used to evaluate the quality of Patrina scabiosaefolia Fisch.

关 键 词：黄花败酱 高效液相色谱评法 灰色关联度分析法 主成分分析 正交偏最小二乘法-判别分析 综合评价 

分 类 号：R284[医药卫生—中药学]