硫利达嗪通过调控miR-3174表达对肝癌Huh-7细胞增殖、迁移和侵袭的影响  

Effect of thioridazine on the proliferation,migration and invasion of liver cancer Huh-7 cells by regulating miR-3174 expression

作　　者：张笑丹[1] 苏江卫[2] 梁倞[3] 梁平[4] Xiao-Dan ZHANG;Jiang-Wei SU;Jing LIANG;Ping LIANG(Department of Pharmacy,Hebei Chest Hospital,Shijiazhuang 050041,China;Department of Clinical Pharmacological Trial,Hebei Chest Hospital,Shijiazhuang 050041,China;Department of Medical Equipment,Hebei Chest Hospital,Shijiazhuang 050041,China;Department of Pharmacy,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北省胸科医院药学部,石家庄050041 [2]河北省胸科医院临床药理试验部,石家庄050041 [3]河北省胸科医院医学装备处,石家庄050041 [4]河北医科大学第四医院药学部,石家庄050011

出　　处：《中国药师》2023年第11期189-196,共8页China Pharmacist

基　　金：河北省中医药管理局科研计划项目(2023310);河北省医学科学研究课题计划(20230877)。

摘　　要：目的 探讨硫利达嗪对肝癌Huh-7细胞生物行为的影响和机制。方法肝癌Huh-7细胞分为对照组及硫利达嗪低、中、高浓度组。转染miR-3174模拟物及其阴性对照、miR-3174抑制物及其阴性对照至Huh-7细胞,并用硫利达嗪处理miR-3174模拟物。CCK-8法和平板克隆实验检测硫利达嗪对Huh-7细胞增殖的影响;划痕愈合实验和Transwell实验检测硫利达嗪对Huh-7细胞迁移和侵袭的影响;实时荧光定量PCR检测硫利达嗪对Huh-7细胞中miR-3174表达的影响。蛋白质印迹法检测E-钙黏蛋白(E-cadherin)和N-钙黏蛋白(N-cadherin)的表达。并通过上述方法检测干扰miR-3174表达或上调miR-3174联合硫利达嗪对Huh-7细胞生物行为的影响。结果硫利达嗪处理和干扰miR-3174表达后Huh-7细胞中miR-3174表达降低,增殖、迁移和侵袭能力及N-cadherin蛋白表达降低,E-cadherin蛋白表达升高(P <0.05)。上调miR-3174表达可减弱硫利达嗪处理对Huh-7细胞恶性生物学行为的影响。结论 硫利达嗪通过下调miR-3174表达可抑制肝癌Huh-7细胞增殖、迁移和侵袭。Objective To study the effect and possible mechanism of thioridazine on the biological behavior of liver cancer Huh-7 cells.Methods Hepatocellular carcinoma Huh-7 cells were randomly divided into a control group and low,medium,and high concentration groups of thiazidine.The miR-3174 mimics and its negative control,miR-3174 inhibitor and its negative control were transfectel to Huh-7 cells,and the miR-3174 mimics and its negative control were treated with thiolidazine.Effect of thioridazine on the proliferation of Huh-7 cells was assessed by CCK-8 method and plate cloning experiment;effect of thioridazine on the migration and invasion of Huh-7 cells was calculated using scratch healing test and Transwell experiment;effect of thioridazine on the expression of miR-3174 in Huh-7 cells was detected by RT-qPCR;E-cadherin and N-cadherin proteins expression was analyzed using Western blotting.The effects of interference with miR-3174 or miR-3174 up-regulation combined with thioridazine on the biological behavior of Huh-7 cells were detected by the above methods.Results After thiolidazine treatment and interference with miR-3174 expression,miR-3174 expression in Huh-7 cells was decreased,proliferation,migration and invasion ability,N-cadherin protein expression were decreased,and E-cadherin protein expression was increased(P<0.05).Up-regulating the expression of miR-3174 attenuated the effect of thiolidazine treatment on the malignant biological behavior of Huh-7 cells.Conclusion Thioridazine inhibits the proliferation,migration and invasion of liver cancer Huh-7 cells by down-regulating miR-3174 expression.

关 键 词：硫利达嗪 肝癌 miR-3174 增殖 迁移 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]