蚯蚓多肽的提取纯化工艺及应用研究进展  

Research Progress in Extraction and Purification Process and Application of Earthworm Polypeptides

作　　者：刘忠慧 周燕代子 王存文[1] LIU Zhonghui;ZHOU Yandaizi;WANG Cunwen(Hubei Key Laboratory of Novel Reactor and Green Chemical Technology,Key Laboratory of Green Chemical Process of Ministry of Education,School of Chemical Engineering and Pharmacy,Wuhan Institute of Technology,Wuhan 430205,China)

机构地区：[1]武汉工程大学化工与制药学院、绿色化工过程教育部重点实验室新型反应器与绿色化学工艺湖北省重点实验室,湖北武汉430205

出　　处：《化学与生物工程》2024年第3期7-14,共8页Chemistry & Bioengineering

基　　金：中国科协第八届青年人才托举工程项目(2022QNRC001);武汉工程大学科学基金项目(k202202);武汉工程大学第十五届研究生教育创新基金项目(CX2023016)。

摘　　要：蚯蚓的蛋白含量极高,经蛋白酶水解可得到不同分子量的多肽类物质,进一步纯化后可得到具有生物活性的小分子蚯蚓多肽。蚯蚓多肽具有平喘降压、抗氧化、增强免疫、抗炎等药理作用。介绍了蚯蚓蛋白的酶解工艺,分析了蚯蚓多肽纯化工艺的优缺点,包括层析法、电泳法、膜分离法等,综述了蚯蚓多肽在神经系统、呼吸系统、心血管系统、免疫系统、运动系统等方面的药理作用,为蚯蚓多肽的开发利用提供了一定的参考。Earthworms have high contents of protein,and polypeptides with different molecular weights can be obtained by proteases hydrolysis.After further purification,small molecular earthworm polypeptides with biological activity can be obtained.Earthworm polypeptides have pharmacological effects,such as relieving asthma,anti-hypertension,antioxidation,enhancing immunity,and anti-inflammation.We introduce the enzymatic hydrolysis process of earthworm protein,and analyze the advantages and disadvantages of the three purification techniques,namely chromatography,electrophoresis,and membrane separation.Moreover,we summarize the pharmacological effects of earthworm polypeptides on the aspects of nervous system,respiratory system,cardiovascular system,immune system,and motor system.This review provides a certain reference for the development and utilization of earthworm polypeptides.

关 键 词：蚯蚓 多肽 水解 纯化 应用 

分 类 号：TQ936.1[化学工程]