应用CRISPR/Cas9技术敲除SLC5A8基因对黑色素瘤的影响  

Effect of knockdown of SLC5A8 gene on melanoma by applying CRISPR/Cas9 technology

作　　者：董玥湘 刘岩 靳小石[3] 刘雅涵[3] 谢天皓 矫政洧[3] DONG Yuexiang;LIU Yan;JIN Xiaoshi;LIU Yahan;XIE Tianhao;JIAO Zhengwei(Hebei University School of Clinical Medicine,Baoding 071000,China;Departement of Emergency Emergency,Xingtai Pepole′s Hospital,Xingtai054000,China;Department of General Surgery,Affiliated Hospital of Hebei University,Baoding071000,China)

机构地区：[1]河北大学临床医学院,保定071000 [2]邢台市人民医院急诊科,邢台054000 [3]河北大学附属医院普通外科,保定071000

出　　处：《天津医科大学学报》2024年第2期128-131,共4页Journal of Tianjin Medical University

基　　金：河北省重点研发计划(21377773D)。

摘　　要：目的:采用CRISPR/Cas9技术研究SLC5A8基因对肿瘤细胞的影响。方法:通过CRISPR/Cas9技术建立SLC5A8基因敲除模型,将C57小鼠分为两组各4只,分别为敲除SLC5A8基因负瘤鼠实验组(KO组)和普通负瘤鼠对照组(WT组)。对比两组的肿瘤时间-生长曲线、肿瘤体积、肿瘤重量及病理学改变。结果:与WT组相比,KO组黑色素瘤生长速度更快,肿瘤体积显著增大(t=7.845,P<0.01)。KO组肿瘤重量较WT组显著增加(t=3.804,P<0.01)。KO组小鼠肿瘤细胞表现出更深层次的异型性,细胞密度不均,结构复杂、形态多样,且存在大量炎细胞浸润。结论:敲除SLC5A8基因影响了C57负瘤鼠黑色素瘤细胞的发展,SLC5A8基因是黑色素瘤进展过程中的负调控因子。Objective:To investigate the effect of the SLC5A8 gene on tumor cells using CRISPR/Cas9 technology.Methods:A CRISPR/Cas9-induced SLC5A8 gene knockout model was created.Eight C57 mice were divided into the knockout group(KO group)with the SLC5A8 gene knocked out in tumor-bearing mice,and the wild-type control group(WT group),with 4 mice in each group.Tumor growth curves,tumor volume,tumor weight,and pathological changes were compared between the two groups.Results:Compared to the WT group,the KO group showed an accelerated melanoma growth rate,a marked increase in tumor volume(t=7.845,P<0.01).Compared with the WT group,the tumor weight significantly increased in mice within the KO group(t=3.804,P<0.01).Tumor cells in KO group showed deeper cellular atypia,uneven cell density,complex and diverse morphology,and abundant infiltration of inflammatory cells.Conclusion:Knocking out the SLC5A8 gene affects the development of melanoma cells in C57 tumor-bearing mice,and the SLC5A8 gene functions as a negative regulator in the progression of melanoma.

关 键 词：CRISPR/Cas9 SLC5A8基因 黑色素瘤 

分 类 号：R73-3[医药卫生—肿瘤]