基于改进AHP-熵权-关联度及化学计量学的经典名方一贯煎提取工艺研究  

Research on extraction process for classical prescription Yiguanjian based on improved analytic hierarchy process-entropy weight-standard decoction relation and chemometrics

作　　者：牛成琳 殷洪梅[1,2,3] 宋明明 周茆 丁涵[1] 徐殿红 陈娅 王振中[1,2,3] 肖伟 NIU Chenglin;YIN Hongmei;SONG Mingming;ZHOU Mou;DING Han;XU Dianhong;CHEN Ya;WANG Zhenzhong;XIAO Wei(Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China;State Key Laboratory on Technologies for Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacture,Lianyungang 222001,China)

机构地区：[1]南京中医药大学,江苏南京210023 [2]江苏康缘药业股份有限公司,江苏连云港222001 [3]中药制药过程控制与智能制造技术全国重点实验室,江苏连云港222001

出　　处：《中草药》2024年第4期1178-1188,共11页Chinese Traditional and Herbal Drugs

基　　金：2022年中央财政转移支付地方项目:基于重点研究室研究领域的中医药多学科研究能力提升项目——中药提取精制新技术。

摘　　要：目的 筛选经典名方一贯煎的最优提取工艺,为其制剂研究提供实验依据。方法 对HPLC法测定的甜菜碱、地黄苷D、对香豆酸、阿魏酸、毛蕊花糖苷、洋川芎内酯Ⅰ进行化学计量学分析;以筛选出的关键成分含量、出膏率及指纹图谱相似度为评价指标,采用改进AHP-熵权法确定各个指标的组合权重系数,以基准样品关联度评价样品与基准样品的质量一致性,在单因素试验的基础上,采用响应面法优选一贯煎的提取工艺参数。结果 Box-Behnken设计建立的数学模型具有统计学意义,一贯煎最佳提取工艺参数为加12倍量水,提取时间80 min,提取2次。此条件下设计空间的综合评分为0.963。结论 采用改进AHP-熵权-基准样品关联度法优选一贯煎提取工艺参数,所拟合的模型显著,与基准样品质量基本一致,为进一步研究经典名方一贯煎复方制剂究提供了参考。Objective The optimal extraction process of classical prescription Yiguanjian(一贯煎)was screened to provide experimental basis for the preparation.Methods The betaine,rehmannioside D,p-coumaric acid,ferulic acid,verbascoside and senkyunolide I,determined by HPLC were screened by chemometrics analysis.With screening of the key ingredient content,extract rate and fingerprint similarity as evaluation indexes,the improved analytic hierarchy process(AHP)-entropy weight method was used to determine the combined weight coefficient of each index,and the quality consistency between the sample and the standard decoction was evaluated by the relation degree of the standard decoction.On the basis of single factor test,the response surface method was used to optimize the extraction process parameters of Yiguanjian.Results The mathematical model established by Box-Behnken experimental design was statistically significant.The optimal extraction process was determined as follows,12 times amount of water,decocting for two times,and each time for 80 min.The comprehensive score of design space was 0.963 under this condition.Conclusion The improved AHP-entropy weight method-standard decoction relation method was used to optimize the extraction process parameters of Yiguanjian,and the fitted model was significant,which was basically consistent with the quality of standard decoction,providing reference for further research of the classical prescription compound preparation of Yiguanjian.

关 键 词：一贯煎 提取工艺 改进AHP 熵权法 基准样品关联度 响应面法 化学计量学 甜菜碱 地黄苷D 对香豆酸 阿魏酸 毛蕊花糖苷 洋川芎内酯Ⅰ 

分 类 号：R283.6[医药卫生—中药学]