低共熔溶剂提取荔枝壳中的原花青素及其抗氧化活性  

Extraction of Litchi Pericarp Procyanidins Using Deep Eutectic Solvents and Their Antioxidant Activity

作　　者：郑德瑜 张瑞芬[2] 黄菲[2] 董丽红[2] 张名位[2] 郭朝万 贾栩超 ZHENG Deyu;ZHANG Ruifen;HUANG Fei;DONG Lihong;ZHANG Mingwei;GUO Chaowan;JIA Xuchao(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;Sericultural&Agri-Food Research Institute Guangdong Academy of Agricultural Sciences,Key Laboratory of Functional Foods,Ministry of Agriculture,Guangdong Key Laboratory of Agricultural Products Processing,Guangzhou 510610,China;Guangdong Marubi Biotechnology Co,Ltd.,Guangzhou 510530,China)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东省农业科学院蚕业与农产品加工研究所,农业农村部功能食品重点实验室,广东省农产品加工重点实验室,广东广州510610 [3]广东丸美生物技术股份有限公司,广东广州510530

出　　处：《现代食品科技》2024年第2期246-256,共11页Modern Food Science and Technology

基　　金：广东特支计划项目(2019BT02N112);广州市科技计划项目(201903010051,202103000055);广东省农业科学院农业优势产业学科团队建设项目(202108TD)。

摘　　要：该研究通过建立低共熔溶剂(DES)最佳提取工艺为荔枝壳原花青素的绿色高效制备提供技术支撑。通过比较了4种低共熔溶剂与70%(V/V)乙醇提取对荔枝壳原花青素提取效率、化学构成及ORAC抗氧化活性的影响,确定氯化胆碱-1,3-丁二醇为最佳提取溶剂。HPLC分析表明4种低共熔溶剂与70%(V/V)乙醇提取的荔枝壳原花青素主要成分均为原花青素A2、芦丁、表儿茶素、原花青素B1及山奈酚-3-O-芸香糖苷。进一步通过单因素试验结合正交优化确定了氯化胆碱-1,3-丁二醇提取最佳工艺条件:料液比1:20(g/mL),溶剂摩尔比1:4,溶剂含水量50%(V/V),提取温度60℃,提取时间90 min,在该条件下提取液中的荔枝壳原花青素含量高达5.07 mg PE/mL,是70%(V/V)乙醇提取的1.4倍,其ORAC值及DPPH和ABTS^(+)自由基的IC_(50)值分别为5496.25μmol TE/mL、27.35μg PE/mL和23.82μg PE/mL,均优于70%(V/V)乙醇提取的原花青素(3370.17μmol TE/mL、36.41μg PE/mL和31.56μg PE/mL)(P<0.05),研究结果表明低共熔溶剂在荔枝壳原花青素提取方面具有显著优势。The optimal process for litchi pericarp procyanidin extraction using deep eutectic solvents(DESs)was established to provide technical support for the efficient green preparation of litchi pericarp procyanidins.Litchi pericarp procyanidins were extracted using four DES types and 70%(V/V)ethanol to investigate and compare the extraction efficiency and the chemical composition and antioxidant activity(oxygen radical absorbance capacity)of the obtained procyanidins.HPLC-DAD analysis revealed that two procyanidin compounds(procyanidin A2 and B1)and three flavonoids(rutin,epicatechin,and kaempferol-3-O-rutinoside)were present in the extracts acquired using the four DESs and ethanol.Choline chloride-1,3-butanediol was the best extractive solvent.Furthermore,single factor tests and orthogonal optimization suggested that the optimum extraction conditions for choline chloride-1,3-butanediol are as follows:a liquid ratio of 1:20(g/mL),a solvent molar ratio of 1:4,a solvent water content of 50%(V/V),an extraction temperature of 60℃,and an extraction time of 90 min.Under these conditions,the total procyanidin content reached 5.07 mg PE/mL,which is 1.4-fold that extracted using 70%ethanol.The oxygen radical absorbance capacity values and the IC_(50) values of the DPPH and ABTS free radicals of the finished choline chloride-1,3-butanediol-extracted extract were 5496.25μmol TE/mL,27.35μg PE/mL,and 23.82μg PE/mL,respectively.In short,these results indicate that DESs exert significant advantages in the extraction of litchi pericarp procyanidins.

关 键 词：荔枝壳 原花青素 低共熔溶剂 抗氧化 

分 类 号：TS209[轻工技术与工程—食品科学]