Revealing the role of CCoAOMT1: fine-tuning bHLH transcription factors for optimal anther development  

作　　者：Zesen Lai Jianzheng Wang Ying Fu Menghan Wang Hong Ma Shiqing Peng Fang Chang 

机构地区：[1]State Key Laboratory of Genetic Engineering,Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering and Institute of Biodiversity Sciences,Institute of Plant Biology,School of Life Sciences,Fudan University,Shanghai 200438,China [2]School of Tropical Agriculture and Forestry,Agriculture-Rural Affairs and Rural Revitalization,Hainan University,Haikou 570228,China [3]Key Laboratory of Biology and Genetic Resources of Tropical Crops,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China [4]Department of Biology,Huck Institutes of the Life Sciences,Pennsylvania State University,University Park,PA 16802,USA

出　　处：《Science China(Life Sciences)》2024年第3期565-578,共14页中国科学（生命科学英文版）

基　　金：supported by the Ministry of Science and Technology,People’s Republic of China(2021YFA0909303);the National Natural Science Foundation of China(32270347,31822005,31870294)。

摘　　要：The tapetum,a crucial innermost layer encompassing male reproductive cells within the anther wall,plays a pivotal role in normal pollen development.The transcription factors (TFs) bHLH010/089/091 redundantly facilitate the rapid nuclear accumulation of DYSFUNCTIONAL TAPETUM 1,a gatekeeper TF in the tapetum.Nevertheless,the regulatory mechanisms governing the activity of bHLH010/089/091 remain unknown.In this study,we reveal that caffeoyl coenzyme A O-methyltransferase 1 (CCoAOMT1) is a negative regulator affecting the nuclear localization and function of bHLH010 and bHLH089,probably through their K259 site.Our findings underscore that CCoAOMT1 promotes the nuclear export and degradation of bHLH010 and bHLH089.Intriguingly,elevated CCoAOMT1 expression resulted in defective pollen development,mirroring the phenotype observed in bhlh010 bhlh089 mutants.Moreover,our investigation revealed that the K259A mutation in the bHLH089 protein disrupted its translocation from the nucleus to the cytosol and impeded its degradation induced by CCoAOMT1.Importantly,transgenic plants with the probHLH089::bHLH089^(K259A)construct failed to rescue proper pollen development or gene expression in bhlh010 bhlh089 mutants.Collectively,these findings emphasize the need to maintain balanced TF homeostasis for male fertility.They firmly establish CCoAOMT1 as a pivotal regulator that is instrumental in achieving equilibrium between the induction of the tapetum transcriptional network and ensuring appropriate anther development.

关 键 词：anther development nuclear localization BHLH O-METHYLTRANSFERASE transcription factor homeostasis 

分 类 号：Q943.2[生物学—植物学]