结肠腺癌组织中DNA-PKcs的表达及临床意义  

Expression and clinical significance of DNA-PKcs in colon adenocarcinoma tissues

作　　者：芮鸿庆 袁立单 刘健 魏翠诊 濮阳永强 周晓华 RUI Hongqing;YUAN Lidan;LIU Jian;WEI Cuizhen;PUYANG Yongqiang;ZHOU Xiaohua(Department of Gastrointestinal Surgery,Nanjing Gaochun People′s Hospital,Nanjing 211300,China)

机构地区：[1]南京市高淳人民医院胃肠外科,南京211300 [2]南京市高淳固城卫生院,211300 [3]南京市高淳人民医院肾内科,211300 [4]南京市高淳人民医院普通外科,211300

出　　处：《临床肿瘤学杂志》2023年第12期998-1003,共6页Chinese Clinical Oncology

摘　　要：目的探讨DNA依赖蛋白激酶催化亚单位(DNA-PKcs)在结肠腺癌(COAD)组织中的表达特征及在COAD预后中的预测价值。方法通过GEPIA在线网站分析275例COAD组织和349例正常对照组织的DNA-PKcs表达;利用Kaplan-Meier Plotter在线网站分析DNA-PKcs表达与COAD患者(1061例)生存情况的关系。选取本院2019年1月至2021年6月行手术切除治疗的58例COAD患者为研究对象,采用免疫组化检测COAD组织中DNA-PKcs表达并分析其与临床病理特征的关系;Pearson相关分析评估DNA-PKcs表达与癌胚抗原(CEA)、糖类抗原199(CA199)的相关性;受试者工作特征(ROC)曲线评估DNA-PKcs、CEA、CA199对COAD不良预后的预测价值。结果在线分析提示COAD组织中DNA-PKcs的表达水平高于正常对照组织(P<0.05),且789例DNA-PKcs低表达者的中位总生存期为134个月,优于272例高表达者的106个月(P<0.05);DNA-PKcs表达在不同TNM分期、分化程度、神经侵袭、浸润深度、复发和淋巴结转移的COAD患者进行比较,差异有统计学意义(P<0.05);DNA-PKcs与CEA、CA199均呈正相关(r=0.431、0.381,均P<0.05);三者联合检测预测COAD不良预后的曲线下面积为0.876(95%CI:0.763~0.948),敏感度为0.862、特异度为0.897。结论DNA-PKcs高表达参与COAD的恶性进展,且其与CEA、CA199联合检测显著提高了对COAD不良预后的预测效能,对COAD不良预后评估具有较高的参考价值。Objective To explore the expression characteristics of protein kinase,DNA-activated,catalytic subunit(DNAPKcs)in colon adenocarcinoma(COAD)tissues and its predictive value in the prognosis of COAD.Methods The expression of DNA-PKcs was analyzed in 275 COAD tissues and 349 normal control tissues through the GEPIA online website.Kaplan-Meier Plotter online website was used to analyze the relationship between DNA-PKcs expression and survival in 1061 COAD patients.Furthermore,58 COAD patients who underwent surgical resection treatment in our hospital from January 2019 to June 2021 were selected as the study subjects.Immunohistochemical detection of DNA-PKcs expression in COAD tissues and analysis of its relationship with clinical pathological features were carried out.Pearson correlation analysis was employed to evaluate the correlation of DNA-PKcs with carcinoembryonic antigen(CEA)and carbohydrate antigen 199(CA199).The predictive value of DNA-PKcs,CEA and CA199 in poor prognosis of COAD was investigated using the receiver operating characteristic(ROC)curve.Results The GEPIA online website showed that the DNA-PKcs expression in COAD tissues was higher than that of the normal controls(P<0.05).The median overall survival of 789 patients with low expression of DNA-PKcs was 134 months,better than the 106 months of 272 patients with high expression(P<0.05).The expression of DNA-PKcs was compared in COAD patients with different TNM stages,differentiation levels,nerve invasion,infiltration depth,recurrence and lymph node metastasis,and the differences were statistically significant(P<0.05).DNA-PKcs was positively correlated with CEA and CA199(r=0.431,0.381,P<0.001).The area under the poor prognosis curve of COAD predicted by the combination of the three indicators was 0.876(95%CI:0.763-0.948),with a sensitivity of 0.862 and a specificity of 0.897.Conclusion High expression of DNA-PKcs is involved in the malignant progression of COAD.The combined detection of DNA-PKcs,CEA and CA199 can significantly improve the predictive e

关 键 词：结肠腺癌 DNA依赖蛋白激酶催化亚单位 预后 诊断 

分 类 号：R735.3[医药卫生—肿瘤]