Effectiveness of Histopathological Examination of Ultrasound-guided Puncture Biopsy Samples for Diagnosis of Extrapulmonary Tuberculosis  

作　　者：GU Wen Fei SHI Xia MA Xin YU Jun Lei XU Jin Chuan QIAN Cheng Cheng HU Zhi Dong ZHANG Hui 

机构地区：[1]Shanghai Public Health Clinical Center,Fudan University,Shanghai 201508,China [2]School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou 325000,Zhejiang,China [3]Department of Laboratory Medicine,Zhejiang Putuo Hospital,Zhoushan 316100,Zhejiang,China [4]Zhongshan Hospital,Fudan University,Shanghai 200032,China

出　　处：《Biomedical and Environmental Sciences》2024年第2期170-177,共8页生物医学与环境科学（英文版）

基　　金：funded by the grants from the National Key Research and Development Program of China[2021YFC2301503,2022YFC2302900];the National Natural and Science Foundation of China[82171739,82171815,81873884]。

摘　　要：Objective To evaluate the diagnostic value of histopathological examination of ultrasound-guided puncture biopsy samples in extrapulmonary tuberculosis(EPTB).Methods This study was conducted at the Shanghai Public Health Clinical Center.A total of 115patients underwent ultrasound-guided puncture biopsy,followed by MGIT 960 culture(culture),smear,Gene Xpert MTB/RIF(Xpert),and histopathological examination.These assays were performed to evaluate their effectiveness in diagnosing EPTB in comparison to two different diagnostic criteria:liquid culture and composite reference standard(CRS).Results When CRS was used as the reference standard,the sensitivity and specificity of culture,smear,Xpert,and histopathological examination were(44.83%,89.29%),(51.72%,89.29%),(70.11%,96.43%),and(85.06%,82.14%),respectively.Based on liquid culture tests,the sensitivity and specificity of smear,Xpert,and pathological examination were(66.67%,72.60%),(83.33%,63.01%),and(92.86%,45.21%),respectively.Histopathological examination showed the highest sensitivity but lowest specificity.Further,we found that the combination of Xpert and histopathological examination showed a sensitivity of 90.80%and a specificity of 89.29%.Conclusion Ultrasound-guided puncture sampling is safe and effective for the diagnosis of EPTB.Compared with culture,smear,and Xpert,histopathological examination showed higher sensitivity but lower specificity.The combination of histopathology with Xpert showed the best performance characteristics.

关 键 词：Extrapulmonary tuberculosis DIAGNOSIS BIOPSY Histopathological examination Puncture samples 

分 类 号：R52[医药卫生—内科学]