脯氨酸对缢蛏高盐耐受性的影响  

Effect of proline on high salt tolerance of razor clam

作　　者：邓敏 刘瑞琦 杨栋 袁立 沈和定[1,2] 牛东红[1,2] DENG Min;LIU Ruiqi;YANG Dong;YUAN Li;SHEN Heding;NIU Donghong(Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产动物遗传育种中心上海市协同创新中心,上海201306 [2]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《上海海洋大学学报》2024年第1期77-85,共9页Journal of Shanghai Ocean University

基　　金：国家重点研发计划“蓝色粮仓科技创新”专项(2019YFD0900700);福建省科技计划项目(2021N3001)。

摘　　要：脯氨酸是生物体内重要的有机调节物质,对海洋贝类适应盐度变化具有重要影响。为了研究脯氨酸对缢蛏(Sinonovacula constricta)高盐耐受性的影响,本研究对缢蛏进行高盐(32)胁迫,并在高盐条件下添加外源氨基酸,对比不同条件处理下缢蛏脯氨酸含量、可溶性蛋白含量以及脯氨酸转运基因表达水平。高盐胁迫下,3种游离氨基酸显著上升,其中,脯氨酸含量积累最多,并始终处于高水平合成状态;可溶性蛋白含量显著降低、排氨率升高;脯氨酸转运基因SLC6A7-1和SLC6A7-2表达显著上升。当添加外源脯氨酸后,脯氨酸含量积累更多,在72 h时达到了峰值;可溶性蛋白含量开始显著增加,排氨水平显著下降;脯氨酸转运基因SLC6A7-1表达先上升后下降,而SLC6A7-2一直处于高表达状态,在24 h时表达量达到峰值。结果表明,脯氨酸可以促进缢蛏体内大分子蛋白合成,为缢蛏高盐适应性生存提供更多能量。本研究为深入探究脯氨酸调控海洋贝类高盐适应性提供了重要理论依据。Proline is an important organic regulatory substance in organisms,which plays an important role in adapting marine shellfish to salinity changes.To study the effect of proline on the salt tolerance of S.constricta,high salt stress and exogenous proline were added to S.constricta.The proline content,soluble protein content and proline transporter gene expression levels of S.constricta were compared in different conditions.Under high salt stress,three free amino acids increased significantly,with the highest proline accumulation and high synthesis.Soluble protein content decreased significantly and ammonia excretion rate increased.The expressions of proline transporter SLC6A7-1 and SLC6A7-2 were significantly increased.When exogenous proline was added,the accumulation of proline content increased and reached the peak at 72 h.Soluble protein content was significantly increased,and ammonia excretion level was significantly decreased.The expression of proline transporter SLC6A7-1 increased firstly and then decreased,while SLC6A7-2 remained in a high expression state and reached its peak at 24 h.The results showed that proline could promote the synthesis of macromolecular protein and provide more energy for the salt-sensitive survival of S.constricta.It provides an important theoretical basis for further study on the regulation of proline in the salt-sensitive survival of marine molluscs.

关 键 词：缢蛏 高盐 脯氨酸 耐受性 

分 类 号：S967.5[农业科学—水产养殖]