杂交链式反应介导赭曲霉毒素A的比色/荧光双模检测  

Colorimetric/fluorescent dual-mode signals for the detection of ochratoxin A mediated by hybridization chain reaction

作　　者：吴家浩 陈丽叶 谢新辉 江紫萍 赵倩 石星波 WU Jiahao;CHEN Liye;XIE Xinhui;JIANG Ziping;ZHAO Qian;SHI Xingbo(Hunan Provincial Key Laboratory of Food Science and Biotechnology,College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学食品科学技术学院,食品科学与生物技术湖南省重点实验室,长沙410128

出　　处：《分析试验室》2024年第2期258-265,共8页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(32001778);湖南农业大学研究生科研创新项目(2022XC050)资助。

摘　　要：以赭曲霉毒素A(OTA)为目标物,以二氧化硅纳米颗粒作为载体,负载赭曲霉毒素适配体和HCR引发链(cDNA)杂交的DNA双链,构建了一种比色/荧光双模信号输出的高灵敏检测平台。在最优条件下,荧光信号回归方程为ΔF=1033.78lgc+13652.89,检出限为6.11×10^(-14)g/mL;比色信号回归方程为A=0.02587lgc+0.7537,检出限为1.33×10^(-13)g/mL。该方法成功应用于实际样品中OTA的检测,为食品安全监测提供了一种新策略。In this study, Ochratoxin A(OTA) as target and silica nanoparticles served as carriers to immobilize DNA double strands hybridized by OTA aptamers and HCR trigger chain reactions(cDNA), a dual-mode colorimetric and fluorescent detection platform with high sensitivity was successfully established. Under the optimum conditions, the fluorescence signal regression equation was ΔF=1033.78lgc+13652.89, along with a detection limit of 6.11×10-^(14) g/mL. And the colorimetric signal regression equation was A=0.02587lgc+0.7537, together with a detection limit of 1.33×10-^(13) g/mL. Furthermore, this method was successfully applied to the detection of OTA in real food samples, offering a new strategy for food safety monitoring.

关 键 词：赭曲霉毒素A 适配体 杂交链式反应 双模信号 金纳米颗粒 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]