甘草酸对高糖诱导的肾小球系膜细胞炎性因子及纤维化因子的影响  

Effect of glycyrrhizin on inflammatory factors and fibrotic factors in high glucose-induced glomerular mesangial cells

作　　者：李媛 王珍[1] 曹雪 侯绍章 Li Yuan;Wang Zhen;Cao Xue;Hou Shao-Zhang(School of Basic Medical College,Ningxia Medical University,Yinchuan,Ningxia 750004,China;School of Nursing,Ningxia Medical University,Yinchuan,Ningxia 750004,China)

机构地区：[1]宁夏医科大学基础医学院,宁夏银川750004 [2]宁夏医科大学护理学院,宁夏银川750004

出　　处：《解放军医学杂志》2024年第2期214-219,共6页Medical Journal of Chinese People's Liberation Army

基　　金：宁夏自然科学基金(2021AAC03125)。

摘　　要：目的探讨甘草酸(GA)对高糖诱导的肾小球系膜细胞(SV40 MES13)炎性因子及纤维化因子的影响。方法将培养的小鼠肾小球系膜细胞(SV40 MES13)分为对照(NG)组(5.6 mmol/L葡萄糖)、高糖(HG)组(30 mmol/L葡萄糖)及HG+GA组(30 mmol/L葡萄糖+200μmol/L GA)。采用Western blotting检测各组细胞炎性因子白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、IL-6、IL-8及纤维化因子α-平滑肌肌动蛋白(α-SMA)蛋白表达水平,免疫荧光检测各组IL-1β、TNF-α、α-SMA在细胞内的荧光表达强度,酶联免疫吸附实验(ELISA)检测各组细胞培养上清中IL-1β、TNF-α、IL-6及IL-8的表达水平。结果Western blotting检测结果显示,与NG组比较,HG组IL-1β、TNF-α、IL-6、IL-8及α-SMA蛋白的表达水平明显升高(P<0.01);与HG组比较,HG+GA组IL-1β、TNF-α、IL-6、IL-8和α-SMA蛋白表达量降低(P<0.05)。细胞免疫荧光结果显示,与NG组比较,HG组细胞内IL-1β、TNF-α和α-SMA因子的荧光强度明显增强(P<0.05);与HG组相比,HG+GA组细胞内IL-1β、TNF-α和α-SMA因子荧光强度明显降低(P<0.05)。ELISA检测结果显示,与NG组比较,HG组细胞培养上清中IL-1β、IL-6、TNF-α和IL-8蛋白表达水平升高(P<0.01);与HG组比较,HG+GA组IL-1β、IL-6、TNF-α、IL-8表达水平明显降低(P<0.05)。结论甘草酸对高糖诱导的肾小球系膜细胞的炎性因子及纤维化因子的表达均具有不同程度的抑制作用,可能在糖尿病肾病的预防方面发挥重要作用。Objective To investigate the effect of glycyrrhizic acid(GA)on the inflammatory and fibrotic factors in high glucose-induced glomerular mesangial cells(SV40 MES13).Methods Cultured mouse SV40 MES13 were divided into normal group(NG,5.6 mmol/L glucose),high glucose group(30 mmol/L glucose)and HG+GA group(30 mmol/L glucose+200μmol/L GA).The expression levels of inflammatory cytokines interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),IL-6,IL-8 andα-smooth muscle actin(α-SMA)in different groups were detected by Western blotting.The fluorescence intensity of IL-1β,TNF-αandα-SMA in different groups were detected by immunofluorescence.The levels of IL-1β,TNF-α,IL-6 and IL-8 in the culture supernatant of different populations were detected by enzyme-linked immunosorbent assay(ELISA).Results The protein expressions of IL-1β,TNF-α,IL-6,IL-8 andα-SMA in HG group were significantly higher than those in NG group(P<0.01);Compared with HG group,the protein expression levels of IL-1β,TNF-α,IL-6,IL-8 andα-SMA decreased significantly in HG+GA group(P<0.05).The fluorescence intensity of inflammatory cytokines IL-1β,TNF-αandα-SMA increased in HG group than those in NG group(P<0.05);While compared with the HG group,the fluorescence intensity of IL-1β,TNF-αandα-SMA in HG+GA group decreased markedly(P<0.05).The experimental results of ELISA showed that compared with NG group,the levels of IL-1β,IL-6,TNF-αand IL-8 in cell supernatent increased in HG group(P<0.01);while the levels of IL-1β,TNF-α,IL-6,IL-8 in HG+GA group significantly lower than those in HG group(P<0.05).Conclusion Glycyrrhizic acid has certain inhibitory effect on high glucoseinduced inflammatory factors and fibrotic factors in glomerular mesangial cells,which may play an important role in prevention of diabetic nephropathy.

关 键 词：糖尿病肾病 高糖 甘草酸 肾小球系膜细胞 炎性因子 纤维化 

分 类 号：R364.2[医药卫生—病理学]