Animal Safety Test of Bacillus thuringiensis BT Protein  

作　　者：Weimeng NING Dingkuo LIU Fang LIU Yuan LI Xiaowei YANG Zhenguo GUAN Guijun YANG Bo ZHANG Pengcheng HU 

机构地区：[1]Dingzheng Xinxing Biotechnology(Tianjin)Co.,Ltd.,Tianjin 300383,China [2]Tianjin Agricultural College,Tianjin 300393,China [3]Tianjin Key Laboratory of Biological Feed Additives,Tianjin 300383,China [4]Tianjin Feed Small Molecule Protein Engineering Center,Tianjin 300383,China [5]Tianjin Agricultural Ecological Environment Monitoring and Agricultural Product Quality Testing Center,Tianjin 300193,China

出　　处：《Asian Agricultural Research》2024年第2期31-32,35,共3页亚洲农业研究（英文）

摘　　要：[Objectives]To determine the biological safety of BT protein from Bacillus thuringiensis(Bt)fermentation broth to mammals at high doses.[Methods]Healthy mice were randomly divided into 4 groups with 10 mice in each group.The experimental groups were fed with Bt fermentation supernatant at 0.2,0.6 and 1.0 mL/kg,respectively,once a day for 7 consecutive days.The blank control group was fed normally without intragastric administration.[Results]There was no significant difference in blood routine and blood biochemical analysis between the experimental group and the control group.After intragastric administration,the mice were dissected,and no obvious pathological changes were found;the heart,liver,spleen,lung and kidney were taken to make tissue sections,and no pathological changes were found by microscopic observation.[Conclusions]Mice can tolerate high doses of BT protein from B.thuringiensis fermentation broth.

关 键 词：Bacillus thuringiensis(Bt) BT protein Biological safety 

分 类 号：TS207.3-4[轻工技术与工程—食品科学] G642[轻工技术与工程—食品科学与工程]