水生动物病原菌Ⅵ型分泌系统(T6SS)及其溶血素共调节蛋白研究进展  

Research progress on typeⅥsecretion system(T6SS)and its hemolysin coregulatory protein from pathogenic bacteria in aquatic animals:a review

作　　者：伍水龙 黄瑜[1,3] 王蓓[1,3] 汤菊芬[1,3] 蔡佳[1,3] 简纪常[1,3] WU Shuilong;HUANG Yu;WANG Bei;TANG Jufen;CAI Jia;JIAN Jichang(College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Clinical Medicine Research Institute of Zhanjiang Central Peoples Hospital,Zhanjiang 524045,China;Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology of Aquatic Economic Animals,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]湛江中心人民医院临床医学研究所,广东湛江524045 [3]广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524088

出　　处：《大连海洋大学学报》2024年第1期162-171,共10页Journal of Dalian Ocean University

基　　金：国家自然科学基金(U20A2065);广东省自然科学基金(2022A1515012203);广东省自然科学基金重大培育项目(2015A030308020)。

摘　　要：Ⅵ型分泌系统(typeⅥsecretion system,T6SS)是多种革兰氏阴性菌编码的蛋白分泌装置,在毒力因子释放、生物被膜形成、铁离子摄取、囊泡运输、环境压力适应性及细菌胞内存活等方面发挥着重要功能,在副溶血弧菌(Vibrio parahaemolyticus)、溶藻弧菌(Vibrio alginolyticus)、哈维氏弧菌(Vibrio harveyi)、嗜水气单胞菌(Aeromonas hydrophila)和迟钝爱德华氏菌(Edwardsiella tarda)等水生动物病原菌的致病过程中发挥着关键作用。然而,有关水生动物病原菌T6SS组分及其功能的报道还比较匮乏。本文对几种水生动物病原菌T6SS的生物学功能与调控机理,以及T6SS关键调控因子溶血素共调节蛋白Hcp的系统进化关系与功能等最新研究情况进行了综述,并在水生动物病原菌T6SS的生物学功能、T6SS活性调控与环境因素的关联性、T6SS与致病菌代谢之间的调控关系等方面提出未来研究建议,以期为进一步开展水生动物致病机制研究及水产养殖细菌病的防控提供新思路。TypeⅥsecretion system(T6SS)as a protein secretion system exists within varieties of gram-negative bacteria,and plays important roles in virulent factors release,biofilm formation,iron ion uptake,vesicular transport and environmental pressure adaptability,which is crucial for the pathogenesis of aquatic bacterial pathogens such as Vibrio parahaemolyticus,V.alginolyticus,V.harveyi,Aeromonas hydrophila,and Edwardsiella tarda.However,the reports regarding the components and functions of T6SS from aquatic pathogenic bacteria are still rare.The latest research progress on biological functions,regulatory mechanisms of T6SS,as well as the biological roles,evolutionary relationships of the key regulator of T6SS-hemolysin coregulatory protein(Hcp)is summarized from several aquatic bacterial pathogens.Several future key directions are proposed including new biofunctional discoveries of T6SS,correlation between T6SS activity and environmental factors,the regulation between T6SS and virulence-related metabolism.This review will help researchers better understand the roles of aquatic animal pathogens T6SS and provide new ideas for further research on pathogenic mechanism and control of bacterial diseases in aquaculture.

关 键 词：Ⅵ型分泌系统 水生动物病原菌 溶血素共调节蛋白 系统进化树 功能位点 

分 类 号：S943[农业科学—水产养殖]