巴西蕉和超级矮蕉中MaGA2ox1基因的克隆和表达模式分析  

Cloning and Expression Pattern Analysis of MaGA2ox1 Gene in Brazilian Banana and Super Dwarf Banana

作　　者：杨医境 程志号 郭素霞 孙长君 王贤智 吴琼 Yang Yijing;Cheng Zhihao;Guo Suxia;Sun Changjun;Wang Xianzhi;Wu Qiong(College of Agriculture,Yunnan University,Kunming,650504;Key Laboratory of Banana Genetic Improvement of Hainan Province,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,57110l;National Key Laboratory for Tropical Crop Breeding,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Sanya,572024)

机构地区：[1]云南大学农学院,昆明650504 [2]中国热带农业科学院热带生物技术研究所,海南省香蕉遗传改良重点实验室,海口571101 [3]中国热带农业科学院热带生物技术研究所,热带作物生物育种全国重点实验室,三亚572024

出　　处：《分子植物育种》2024年第6期1834-1841,共8页Molecular Plant Breeding

基　　金：海南省自然科学基金高层次人才项目(320RC684)资助。

摘　　要：为了培育抗倒伏矮秆香蕉,本研究以常规栽培种巴西蕉(Musa spp. Baxi, BX)和超级矮蕉(super dwarf cavendish, SDC)为材料,对二者GA2ox基因进行克隆、荧光定量表达和烟草亚细胞定位分析发现,该基因cDNA全长990 bp,编码329个氨基酸,其编码蛋白的氨基酸序列在这两种材料中完全一致;蛋白分子量为36.4 k D,等电点为6.27,无跨膜区和信号肽;MaGA2ox1基因所合成的蛋白质序列与椰子、水稻、拟南芥的亲缘关系最为密切;该基因定位于在细胞膜和细胞核中;五叶一心期,巴西蕉的根、叶片、心叶等组织中MaGA2ox1基因表达均高于SDC;十叶一心期,巴西蕉的老叶和根组织中的MaGA2ox1基因表达量高于SDC,在嫩叶和心叶组织中MaGA2ox1基因表达量低于SDC,两时期MaGA2ox1基因的总组织表达量巴西蕉高于SDC。本研究为解析香蕉形态建成过程中GA2ox氧化酶的调控机制提供一定的参考。In order to cultivate lodging-resistant dwarf bananas,this study took Brazil banana(Musa spp.Baxi,BX)and super dwarf cavendish(SDC),which were commonly cultivated as materials,cloned the GA 2ox gene,fluorescence quantitative expression and tobacco subcellular localization analysis.The cDNA length of this gene is 990 bp,encoding 329 amino acids,and the amino acid sequence of its encoded protein is completely consistent in the two materials.The molecular weight of the protein is 36.4 kD,the isoelectric point is 6.27,and there is no transmembrane region and signal peptide.The protein sequence synthesized by MaGA 2ox I gene is most closely related to coconut,rice and Arabidopsis.The gene is localized in cell membrane and nucleus.At the five-leaf stage,the expression of MaGA2oxl gene in roots,leaves and heart leaves of SDC was significantly lower than that of Brazil banana.At the ten-leaf stage,the expression of MaGA2oxl gene in the heart leaves of SDC was significantly lower than that of Brazil banana.The total tissue expression of MaGA2oxl gene in banana was higher than that in SDC.In this study,by analyzing the subcellular localization and expression pattern of MaGA2oxl gene in Brazil banana and SDC,it provides a reference for studying the regulation of GA synthesis and the role of GA signal transduction during banana development,and the breeding of dwarf banana varieties.

关 键 词：香蕉 GA2ox1 系统进化树 亚细胞定位 表达模式分析 

分 类 号：S668.1[农业科学—果树学]