层积条件和激素处理对朝鲜淫羊藿种子后熟的影响  

Effects of Stratification Conditions and Hormone Treatments on Seed Post-ripening of Epimedium koreanum Nakai

作　　者：张明月 王蕾[1] 王克凤 董然[1] Zhang Mingyue;Wang Lei;Wang Kefeng;Dong Ran(College of Horticulture,Jilin Agricultural University,Changchun,130118;Changbai Mountain Ecological Resources Development Engineering Research Center,Changchun University of Science and Technology,Changchun,130600)

机构地区：[1]吉林农业大学园艺学院,长春130118 [2]长春科技学院,长白山生态资源开发工程研究中心,长春130600

出　　处：《分子植物育种》2024年第6期2044-2051,共8页Molecular Plant Breeding

基　　金：吉林农业大学校内启动基金项目(优质特色寒地花卉资源评价与品种创制)-博士启动基金(202023298)资助。

摘　　要：为探明朝鲜淫羊藿种子后熟机理及解决种子育苗难问题,以其6至7成熟种子为试验材料,研究比较自然层积和4℃低温层积两种条件下种子完成后熟情况,并进一步筛选促进种胚生长的激素浓度。结果表明:自然层积种子率先完成后熟,后熟时间最短为80 d,层积至120 d时胚长达2.703 3 mm、胚宽达0.76 mm、胚率达70.33%,三项指标均显著优于低温层积;赤霉素(GA3) 400 mg/L为自然层积条件下最佳激素浓度,层积至120 d时其胚率达88.23%,低温层积条件下各外源激素均不能明显促进种胚发育。综上,种子采后经自然层积120 d能顺利完成种胚后熟过程,配合使用赤霉素(GA3) 400 mg/L效果更好。该研究为朝鲜淫羊藿种子后熟及萌发机制提供理论依据。In order to investigate the mechanism of seed post-ripening in Epimedium koreanum Nakai and to solve the problem of seed breeding,seed post-ripening conditions under natural stratification and low temperature stratification were compared and the concentrations of hormones promoting embryo growth were further screened by using six to seven percentage mature seeds.The results showed that:under natural stratification(A),the seeds were first finished post-ripening,the shortest post-ripening time was 80 days;when stratification reached 120 days,the embryo length was 2.7033 mm,the embryo width was 0.76 mm and the embryo rate was 70.33%,which was significantly better than low temperature stratification;under natural stratification the best concentration of gibberellin(GA,)was 400 mg/L,and the embryo rate reached 88.23%after 120 days of stratification.Under low temperature stratification,none of the exogenous hormones could obviously promote the development of seed embryos.In conclusion,the post-ripening process of seed embryos could be finished successfully after 120 days of natural stratification,and the effect of GA,400 mg/L is better,this study provided a theoretical basis for post-ripeningand seed germination mechanism of Epimedium koreanum Nakai.

关 键 词：朝鲜淫羊藿 种子后熟 层积条件 激素 

分 类 号：S567.239[农业科学—中草药栽培]