Exercised blood plasma promotes hippocampal neurogenesis in the Alzheimer's disease rat brain  

作　　者：Cecilie Skarstad Norevik Aleksi M.Huuha Ragnhild N.RФsbjФrgen Linda Hildegard Bergersen Kamilla Jacobsen Rodrigo Miguel-dos-Santos Liv Ryan Belma Skender Jose Bianco N.Moreira Asgeir Kobro-Flatmoen Menno P.Witter Nathan Scrimgeour Atefe R.Tari 

机构地区：[1]Cardiac Exercise Research Group(CERG),Department of Circulation and Medical Imaging,Norwegian University of Science and Technology,7491,Trondheim,Norway [2]Department of Neurology and Clinical Neurophysiology,St.Olavs University Hospital,7030,Trondheim,Norway [3]Institute of Oral Biology,University of Oslo,0372,Oslo,Norway [4]Department of Physiology,Federal University of Sergipe,S~ao Cristov~ao,49100-000,Sergipe,Brazil [5]Centre of Molecular Inflammation Research,Department of Clinical and Molecular Medicine,Norwegian University of Science and Technology,7491,Trondheim,Norway [6]Kavli Institute for Systems Neuroscience,Centre for Neural Computation,and Egil and Pauline Braathen and Fred Kavli Centre for Cortical Microcircuits,Norwegian University of Science and Technology,7030,Trondheim,Norway [7]K.G.Jebsen Centre for Alzheimer’s Disease,Norwegian University of Science and Technology,7030,Trondheim,Norway

出　　处：《Journal of Sport and Health Science》2024年第2期245-255,共11页运动与健康科学（英文）

基　　金：funded by The Norwegian Research Council,the Liaison Committee between the Central Norway Regional Health Authority;funded by the Coordination for the Improvement of Higher Education PersonnelBrazil(Capes)。

摘　　要：Background:Exercise training promotes brain plasticity and is associated with protection against cognitive impairment and Alzheimer’s disease(AD).These beneficial effects may be partly mediated by blood-borne factors.Here we used an in vitro model of AD to investigate effects of blood plasma from exercise-trained donors on neuronal viability,and an in vivo rat model of AD to test whether such plasma impacts cognitive function,amyloid pathology,and neurogenesis.Methods:Mouse hippocampal neuronal cells were exposed to AD-like stress using amyloid-βand treated with plasma collected from human male donors 3 h after a single bout of high-intensity exercise.For in vivo studies,blood was collected from exercise-trained young male Wistar rats(high-intensity intervals 5 days/week for 6 weeks).Transgenic AD rats(McGill-R-Thyl-APP)were inj ected 5 times/fortnight for 6 weeks at2 months or 5 months of age with either(a)plasma from the exercise-trained rats,(b)plasma from sedentary rats,or(c)saline.Cognitive function,amyloid plaque pathology,and neurogenesis were assessed.The plasma used for the treatment was analyzed for 23 cytokines.Results:Plasma from exercised donors enhanced cell viability by 44.1%(p=0.032)and reduced atrophy by 50.0%(p<0.001)in amyloid-β-treated cells.In vivo exercised plasma treatment did not alter cognitive function or amyloid plaque pathology but did increase hippocampal neurogenesis by~3 fold,regardless of pathological stage,when compared to saline-treated rats.Concentrations of 7 cytokines were significantly reduced in exercised plasma compared to sedentary plasma.Conclusion:Our proof-of-concept study demonstrates that plasma from exercise-trained donors can protect neuronal cells in culture and promote adult hippocampal neurogenesis in the AD rat brain.This effect may be partly due to reduced pro-inflammatory signaling molecules in exercised plasma.

关 键 词：CYTOKINES High-intensity interval training INFLAMMATION NEURONS Plasma transfusion 

分 类 号：G804.2[文化科学—运动人体科学]