基于循证医学方法对眼底病专病生物样本库建设的分析与启示  

Construction of fundus disease-specific biobank: an evidence-based analysis and inspiration

作　　者：古秋梅 陈正举 陆方[1] Gu Qiumei;Chen Zhengju;Lu Fang(Department of Ophthalmology,West China Hospital,Sichuan University,Chengdu 610041,China;Department of Biobank,West China Hospital,Sichuan University,Chengdu 610041,China;Science and Technology Department,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川大学华西医院眼科,成都610041 [2]四川大学华西医院生物样本库,成都610041 [3]四川大学华西医院科技部,成都610041

出　　处：《中华眼底病杂志》2024年第3期215-221,共7页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金区域联合项目(U22A20311)。

摘　　要：目的探讨眼底病专病生物样本库建设的必要性、建设方案及实施办法。方法循证医学研究。以“生物样本库”、“专病库”、“眼病”、“眼底病”、“Disease-specific biobank”、“Biobank”、“Eye disease”、“Construction”、“Fundus disease”为中英文检索词,在美国国立医学图书馆医学数据库PubMed、荷兰医学文摘数据库Embase、中国知网和万方科技期刊全文数据库,检索建库至2023年10月与专病生物样本库建设相关的文献,由两名研究者独立筛选文献、提取资料后进行分析。结果经过筛选共纳入23篇文献,其中中文、英文文献分别为11、12篇,涉及23个机构。专病生物样本库:建设时间:国外较国内稍早。规模:国内外均存在<1、1~10、>10万份的专病库,其中眼病专病库存量<1万份;均以肿瘤类疾病居多;均由实体库和信息库组成;样本库建设均要求伦理委员会申报备案;均设有专职人员;均建立有信息管理系统;质控体系上均制定了样本采集至入库的标准操作流程;均能有效支撑科研项目。推进资源共享:国内较国外欠缺。结合实际经验,四川大学华西医院生物样本库对眼底病专病生物样本库的建设进行了完善,分别制定了门诊和手术室样本采集流程,对于眼科特有的样本,包括玻璃体液、视网膜增生膜、房水、泪液等均制定了标准操作流程,以确保样本质量。结论建立眼底病专病样本库可促进眼底病基础和临床研究,推进转化医学发展;实现资源共享,促进学科发展。Objective To explore the necessity,construction plans,and implementation methods for the establishment of a fundus disease-specific biobank are discussed.Methods An evidence-based medicine study.“Biobank”,“Disease-specific biobank”,“Eye diseases”,“Fundus disease”were hereby used as search terms.Literatures were retrieved related to biobank construction from PubMed,Embase,China National Knowledge Infrastructure,and Wanfang databases since their establishment until October 2023.Two researchers independently selected and analyzed literature,extracting data for further analysis.Results After screening,23 articles were included,comprising 11 articles in Chinese and 12 articles in English,involving 23 institutions.The disease-specific biobank has been built earlier abroad than domestically.Both domestic and foreign biobank have a scale of less than 1,1 to 10,and more than 10 thousand samples,with the eye disease-specific biobank having less than 10 thousand samples.The majority of these disease-specific biobank focued on tumor-related diseases and consist of both physical and information components.Ethical committees were required to declare and record the construction of biobank,and dedicated personnel and information management systems are established.Quality control systems have been developed with standard operating procedures from sample collection to storage.These disease-specific biobank effectively supported research projects,but there was a lack of resource sharing domestically compared to abroad.Based on practical experience,the construction of fundus disease-specific biobank at West China Hospital of Sichuan University has been improved. Separate samplecollection processes have been formulated for outpatient clinics and operating rooms. Standard operatingprocedures have been established for unique eye samples, including vitreous fluid, proliferative membranes ofthe retina, aqueous humor, tears, etc., to ensure sample quality. Conclusion Establishing a fundus diseasespecificbiobank can promote

关 键 词：生物样本库 专病库 眼底病 循证医学 

分 类 号：R77[医药卫生—眼科]