单细胞转录组学在眼底疾病领域的研究进展  

Research progress of single cell transcriptomics in ocular fundus diseases

作　　者：茅希颖[1] 殷文婕 徐淼 袁松涛[1] 刘庆淮[1] Mao Xiying;Yin Wenjie;Xu Miao;Yuan Songtao;Liu Qinghuai(Department of Ophthalmology,The First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学第一附属医院眼科,南京210029

出　　处：《中华眼底病杂志》2024年第3期243-246,共4页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金(82271100、82201239)。

摘　　要：单细胞测序技术为解析生物组织的细胞异质性提供了强有力的技术支持,现已广泛应用于生物医学领域。近年来,关于眼底疾病的研究也有了较可观的单细胞数据积累。眼底遍布丰富的血管与神经网络,这导致眼底疾病发病机制纷繁复杂。通过单细胞测序技术能够获得疾病环境下某种细胞类型甚至亚型的成千上万个基因表达情况。单细胞测序技术精准揭示了新生血管性眼病等眼底疾病的致病细胞类型及致病机制,为新型诊疗靶点的诞生提供了理论基础。眼底疾病多维组学单细胞数据库的构建将使得高质量数据能够得到更加深入的探究,为眼科科研工作者提供分析平台。Single cell RNA sequencing technique provides a strong technical support for the analysis of cell heterogeneity in biological tissues,and has been widely used in biomedical research.In recent years,considerable scRNA-seq data have been accumulated in the research of ocular fundus diseases.The ocular fundus is abundant for the network of vessel and neuron,which leads to the complicated pathogenesis of fundus diseases.Through single cell RNA sequencing technique,the expression of thousands of genes of certain cell types or even subtypes can be obtained in the disease environment.Single cell RNA sequencing technique accurately reveals the pathogenic cell types and pathogenic mechanisms of ocular fundus diseases such as neovascular retinopathy,which provides a theoretical basis for the birth of new diagnosis and treatment targets.The construction of multi-omics single-cell database of ocular fundus diseases will enable high-quality data to be further explored and provide an analysis platform for ophthalmic researchers.

关 键 词：单细胞测序技术 眼底疾病 共享数据库 综述 

分 类 号：R77[医药卫生—眼科]