Alisol B 23-acetate promotes white adipose tissue browning to mitigate high-fat diet-induced obesity by regulating mTOR-SREBP1 signaling  

作　　者：Lu-lu Han Xin Zhang Hui Zhang Ting Li Yi-chen Zhao Ming-hui Tian Feng-lei Sun Bo Feng 

机构地区：[1]Department of Neurology Three,The Fifth People’s Hospital of Jinan,Jinan 250013,Shandong Province,China [2]Department of Gastroenterology,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong Province,China [3]The First Clinical Medical College,Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong Province,China [4]Department of Geriatrics,the First Affiliated Hospital of Shandong First Medical University(Shandong Provincial Qianfoshan Hospital),Jinan 250014,Shandong Province,China [5]Chinese Medicine Culture and Literature Research Institute,Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong Province,China [6]Department of General Medicine,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong Province,China [7]Department of Traditional Chinese Medicine,the Second People’s Hospital of Haibei Prefecture,Zangzu Autonomous Prefecture of Haibei,810300,Qinghai Province,China

出　　处：《Journal of Integrative Medicine》2024年第1期83-92,共10页结合医学学报（英文版）

基　　金：supported by Shandong Provincial Natural Science Foundation General Program(No.ZR2022MH213);Shandong Provincial Traditional Chinese Medicine Science and Technology Project General Program(No.M2023241);Jinan Clinical Medical Science and Technology Innovation Program(No.202328013);Qinghai Province High-end Innovative Talents Thousand Talents Program.

摘　　要：Objective Obesity is a global health concern with management strategies encompassing bariatric surgery and anti-obesity drugs;however,concerns regarding complexities and side effects persist,driving research for more effective,low-risk strategies.The promotion of white adipose tissue(WAT)browning has emerged as a promising approach.Moreover,alisol B 23-acetate(AB23A)has demonstrated efficacy in addressing metabolic disorders,suggesting its potential as a therapeutic agent in obesity management.Therefore,in this study,we aimed to investigate the therapeutic potential of AB23A for mitigating obesity by regulating metabolic phenotypes and lipid distribution in mice fed a high-fat diet(HFD).Methods An obesity mouse model was established by administration of an HFD.Glucose and insulin metabolism were assessed via glucose and insulin tolerance tests.Adipocyte size was determined using hematoxylin and eosin staining.The expression of browning markers in WAT was evaluated using Western blotting and quantitative real-time polymerase chain reaction.Metabolic cage monitoring involved the assessment of various parameters,including food and water intake,energy metabolism,respiratory exchange rates,and physical activity.Moreover,oil red O staining was used to evaluate intracellular lipid accumulation.A bioinformatic analysis tool for identifying the molecular mechanisms of traditional Chinese medicine was used to examine AB23A targets and associated signaling pathways.Results AB23A administration significantly reduced the weight of obese mice,decreased the mass of inguinal WAT,epididymal WAT,and perirenal adipose tissue,improved glucose and insulin metabolism,and reduced adipocyte size.Moreover,treatment with AB23A promoted the expression of browning markers in WAT,enhanced overall energy metabolism in mice,and had no discernible effect on food intake,water consumption,or physical activity.In 3T3-L1 cells,AB23A inhibited lipid accumulation,and both AB23A and rapamycin inhibited the mammalian target of rapamycin-sterol regulato

关 键 词：OBESITY Alisol B 23-acetate Adipose tissue mTOR-SREBP1 High-fat diet 

分 类 号：R285[医药卫生—中药学]