抑制hsa_circRNA6448-14表达对人食管鳞状细胞癌细胞系KYSE30及KYSE150侵袭迁移的影响  

Effects of inhibiting expression of hsa_circRNA6448-14 on invasion and migration of human squamous cell carcinoma cell lines KYSE30 and KYSE150

作　　者：王平[1,2,3,4] 李梦辉 张耀文 WANG Ping;LI Menghui;ZHANG Yaowen(不详;School of Clinical Medicine,Henan University of Science and Technology,Luoyang 471003,China)

机构地区：[1]河南科技大学临床医学院放疗科,河南洛阳471003 [2]河南科技大学第一附属医院 [3]安阳市肿瘤医院,河南科技大学附属安阳肿瘤医院放疗科 [4]河南省食管癌精准防治医学重点实验室

出　　处：《山东医药》2024年第6期6-9,共4页Shandong Medical Journal

基　　金：河南省卫健委杰青人才项目(YXKC2021045);河南省科技攻关项目(212102310702);安阳市科技攻关项目(2021C01SF011)。

摘　　要：目的观察抑制环状RNA(circular RNA,circRNA)hsa_circRNA6448-14表达对人食管鳞状细胞癌(ES‐CC)细胞系KYSE30及KYSE150侵袭、迁移的影响,证实hsa_circRNA6448-14在ESCC中的生物学功能。方法体外传代培养KYSE30及KYSE150细胞。随机分为KYSE30敲降组、KYSE150敲降组、KYSE30对照组及KYSE150对照组,KYSE30敲降组和KYSE150敲降组转染hsa_circRNA6448-14-siRNA干扰质粒(抑制hsa_circRNA6448-14表达),KYSE30对照组及KYSE150对照组加入siNC质粒(空白质粒)转染,培养48 h时采用qRT-PCR检测各组细胞hsa_circRNA6448-14,成功培养抑制hsa_circRNA6448-14表达的KYSE30及KYSE150细胞。培养24 h时采用Tran‐swell实验观察各组细胞侵袭情况、采用划痕实验观察各组细胞迁移情况。结果培养24 h时KYSE30敲降组、KYSE30对照组、KYSE150敲降组及KYSE150对照组穿膜细胞数分别为58.00±3.61、161.33±4.06、69.00±2.08、191.33±6.39;与对照组比较,培养24 h时敲降组细胞穿模细胞数小(t分别为19.04、18.21,P均<0.05)。培养24 h时KYSE30敲降组、KYSE30对照组、KYSE150敲降组及KYSE150对照组细胞迁移面积分别为(11.67±0.88)、(26.00±1.73)、(14.33±0.88)、(28.00±1.53)mm2;与对照组比较,培养24 h时敲降组细胞迁移面积小(t分别为7.37、7.75,P均<0.05)。结论抑制hsa_circRNA6448-14表达的KYSE30及KYSE150细胞侵袭、迁移能力降低。hsa_circRNA6448-14在ESCC的侵袭及迁移过程中发挥重要作用。Objective To investigate the effects of inhibiting the expression of circular RNA(circRNA)hsa_cir‐cRNA6448-14 on the invasion and migration of human esophageal squamous cell carcinoma(ESCC)cell lines KYSE30 and KYSE150 and to confirm the biological function of hsa_circRNA6448-14 in ESCC.Methods In vitro passage cul‐ture of KYSE30 and KYSE150 cells was conducted.The cells were randomly divided into the KYSE30 knockdown group,KYSE150 knockdown group,KYSE30 control group,and KYSE150 control group,respectively.Cells in the KYSE30 knockdown group and KYSE150 knockdown group were transfected with hsa_circRNA6448-14-siRNA interference plasmid(inhibiting hsa_circRNA6448-14 expression),while cells in the KYSE30 control group and KYSE150 control group were transfected with siNC plasmid(blank plasmid).After 48 h of culture,qRT-PCR was used to detect the expression of hsa_circRNA6448-14 in each group,and we successfully cultured KYSE30 and KYSE150 cells with inhibited hsa_cir‐cRNA6448-14 expression.The invasion and migration abilities of cells in each group were detected by Transwell experi‐ment and Scratch assay after 24 h of culture.Results The number of transmembrane cells in the KYSE30 knockdown group,KYSE30 control group,KYSE150 knockdown group,and KYSE150 control group after 24 h of culture were 58.00±3.61,161.33±4.06,69.00±2.08,and 191.33±6.39,respectively.Compared with the control groups,the number of transmembrane cells in the knockdown groups was significantly smaller(t=19.04,18.21,respectively;all P<0.05).The migration area of cells in the KYSE30 knockdown group,KYSE30 control group,KYSE150 knockdown group,and KYSE150 control group after 24 h of culture was(11.67±0.88),(26.00±1.73),(14.33±0.88),and(28.00±1.53)mm2,respectively.Compared with the control groups,the knockdown groups showed significantly smaller migration area after 24 h of culture(t=7.37,7.75,respectively;all P<0.05).Conclusions Inhibition of hsa_circRNA6448-14 ex‐pression reduces the invasive and migratory abilities of KYSE30 and KY

关 键 词：环状RNA hsa_circRNA6448-14 食管肿瘤 食管癌 食管鳞癌 细胞侵袭 细胞迁移 

分 类 号：R735.1[医药卫生—肿瘤]