基于气相色谱-质谱研究肝喜片处方中挥发性成分  

Study on the volatile components in Ganxi tablets based on GC-MS

作　　者：陈玲珑 陆艳艳[3] 彭艳梅[1,2] CHEN Lingong;LU Yanyan;PENG Yanmei(Hunan University of Chinese Medicine,Changsha,410208,Hunan,China;Hunan Academy of Chinese Medicine,Chang-sha,410006,Hunan,China;Institute of Acoustics,Chinese Academy of Sciences,Beijing,100190,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省中医药研究院,湖南长沙410006 [3]中国科学院声学研究所,北京100190

出　　处：《肿瘤药学》2024年第1期59-64,共6页Anti-Tumor Pharmacy

基　　金：长沙市科技局项目(kq2004047)。

摘　　要：目的前期研究显示肝喜片处方中陈皮、茵陈、沉香等6种含挥发性成分中药饮片提取的芳香水中解析出8种挥发性成分,其中柠檬烯、γ-萜品烯、冰片、β-蒎烯具有抗炎、抗氧化、抗肿瘤等作用。为进一步研究肝喜片全方中挥发性成分,本研究采用气相色谱-质谱法(GC-MS)分析医院制剂肝喜片处方中的挥发性化学成分。方法利用水蒸气蒸馏法提取肝喜片中非全方芳香水及全方芳香水,并结合GC-MS技术对其成分进行检测及解析。结果肝喜片全方中药饮片提取的芳香水中共检测出28种挥发性成分,含量较高的成分为右旋樟脑(48.45%)、苯甲醛(12.46%)、芳香酮(8.69%),合计69.6%,但均无明显药理作用;其他少量成分有药理作用,但含量很低。结论肝喜片工艺优化提取挥发性成分时不宜用全方,应优选陈皮、茵陈、沉香等6种含挥发性成分中药饮片提取芳香水。Objective Previous studies showed that eight volatile components were separated from aromatic water which were extracted from 6 kinds of Chinese herbal pieces(including tangerine peel,herba artemisiae scopariae and others)in the prescription of Ganxi tablet.Of the eight volatile components,limonene,γ-terpene,borneol andβ-pinene had anti-in-flammatory,anti-oxidation and anti-tumor effects.Here,we aimed to analyze the volatile constituents of all the Chinese herbal pieces in the prescription of Ganxi tablet by gas chromatography-mass spectrometry(GC-MS).Methods The aro-matic water was extracted from Ganxi tablet by steam distillation,and its components were analyzed by GC-MS.Results A total of 28 volatile components were detected in the aromatic water extracted from all the Chinese herbal pieces in Ganxi tablet.The highest content component was D-camphor(48.45%),followed by benzaldehyde(12.46%)and aromatic ketone(8.69%),with a total of 69.6%,but none of them had significant pharmacological effects.A few other components had phar-macological effects,but in very low content.Conclusion It is not suitable to use the whole formula when extracting the vola-tile components of Ganxi tablet.It is preferred to choose the 6 kinds of Chinese herbal pieces like tangerine peel,herba ar-temisiae scopariae,agarwood and others which contain volatile components to extract aromatic water.

关 键 词：肝喜片 芳香水 挥发性成分 气相色谱-质谱法 

分 类 号：R917[医药卫生—药物分析学]