彩色多普勒超声检查联合血清miR-122-5p、miR-106b水平对卵巢癌的诊断价值  

Diagnostic value of color Doppler ultrasonography combined with serum miR-122-5p and miR-106b levels in ovarian cancer

作　　者：张纪妍 高芳圆[1] 颜瑞雪 苗瑞宁 肖喜云 奚杰[1] ZHANG Ji-yan;GAO Fang-yuan;YAN Rui-xue(Department of Obstetrics and Gynecology,Cangzhou Central Hospital,Cangzhou Hebei 061000,China)

机构地区：[1]沧州市中心医院妇科,河北沧州061000

出　　处：《临床和实验医学杂志》2024年第4期429-433,共5页Journal of Clinical and Experimental Medicine

基　　金：沧州市重点研发计划指导项目(编号:222106032);河北省2022年度医学科学研究课题计划项目(编号:20220341)。

摘　　要：目的 探讨彩色多普勒超声检查联合血清微小RNA(miRNA)-122-5p、miR-106b水平对卵巢癌的早期诊断价值以及与卵巢癌周围血管侵袭的相关性。方法 回顾性选取2019年5月至2021年12月沧州市中心医院收治的102例卵巢肿瘤患者为研究对象,所有患者均行彩色多普勒超声检查,采用实时定量聚合酶链反应测定血清中miR-122-5p和miR-106b的表达水平。以病理诊断结果作为金标准,将研究对象分为卵巢癌组(n=51)和良性卵巢肿瘤组(n=51);卵巢癌组又分为侵袭组(n=32)和非侵袭组(n=19)。比较卵巢癌组与良性卵巢肿瘤组的临床资料(年龄、体重指数、生育和绝经)、彩色多普勒超声图像特征(实性包块、伴腹水、乳头状结构≥4、肿瘤最大直径>10 cm)、彩色多普勒超声血流参数[搏动指数(PI)和血流阻力指数(RI)]、血清miR-122-5p、miR-106b水平。应用多因素Logistic回归模型探究相关变量对卵巢癌的独立预测作用。应用受试者工作特征(ROC)曲线评估彩色多普勒超声检查联合血清miR-122-5p、miR-106b水平对卵巢癌的诊断价值。并比较侵袭组与非侵袭组彩色多普勒超声血流参数及血清miR-122-5p、miR-106b水平,采用Pearson相关性分析对彩色多普勒超声血流参数、miR-122-5p、miR-106b与卵巢癌周围血管侵袭的相关性进行分析。结果 卵巢癌组实性包块、伴腹水、乳头状结构≥4以及肿瘤最大直径>10 cm所占比例分别为80.39%、76.47%、74.51%、78.43%,均高于良性卵巢肿瘤组(9.80%、11.76%、29.41%、45.10%),PI、RI、血清miR-122-5p表达水平分别为0.94±0.27、0.48±0.11、1.01±0.27,均低于良性卵巢肿瘤组(1.47±0.32、0.71±0.19、1.42±0.30),miR-106b表达水平为4.57±1.13,高于良性卵巢肿瘤组(2.86±0.65),差异均有统计学意义(P<0.05)。Logistic回归模型分析显示,PI、RI、miR-122-5p、miR-106b是卵巢癌的独立预测因子,OR值分别为1.080、1.116、1.689、1.301(P<0.05)。ROC曲线分析Objective To investigate the value of color Doppler ultrasonography combined with serum microRNA(miRNA)-122-5p and miR-106b levels in the early diagnosis of ovarian cancer and its correlation with vascular invasion around ovarian cancer.Methods A total of 102 patients with ovarian tumors admitted to Cangzhou Central Hospital from May 2019 to December 2021 were retrospectively selected as the research objects.All patients underwent color Doppler ultrasonography.The expression levels of miR-122-5p and miR-106b in serum were determined by real-time quantitative polymerase chain reaction.The subjects were divided into ovarian cancer group(n=51) and benign ovarian tumor group(n=51) with pathological diagnosis as the gold standard.The ovarion cancer group was divided into invasive group(n=32) and non-invasive group(n=19).Clinical data(age,body mass index,fertility,and menopause),color Doppler ultrasound image features(solid mass,accompanied by ascites,papillary structure≥4,maximum tumor diameter>10 cm),color Doppler ultrasound blood flow parameters[(pulsatility index(PI) and blood flow resistance index(RI)],serum miR-122-5p and miR-106b levels were compared between the ovarian cancer group and the benign ovarian tumor group.Multiple Logistic regression model was used to explore the independent predictive effect of related variables on ovarian cancer.The receiver operating characteristic curve(ROC) was used to evaluate the diagnostic value of color Doppler ultrasound combined with serum miR-122-5p and miR-106b levels in ovarian cancer.Color Doppler ultrasound blood flow parameters and serum miR-122-5p and miR-106b levels were compared between the invasive and non-invasive groups.The correlation between color Doppler ultrasound blood flow parameters,miR-122-5p,miR-106b and vascular invasion around ovarian cancer was analyzed using Pearson correlation analysis.Results The proportions of solid masses,ascites,papillary structures≥4,and maximum tumor diameter>10 cm in the ovarian cancer group was 80.39%,76.47%,74.51%,and

关 键 词：彩色多普勒超声 微小RNA miR-122-5p miR-106b 卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤] R445.1[医药卫生—临床医学]