基于GEO数据库探讨UBE2T在肺腺癌中的作用  

Investigating the role of UBE2T in lung adenocarcinoma based on GEO database

作　　者：李晶[1] 汪巍[2] 彭敦钰[1] LI Jing;WANG Wei;PENG Dunyu(Department of Anesthesia,People’s Hospital of Wuhan University,Wuhan 430060,Hubei,China;Department of Thoracic Surgery,People’s Hospital of Wuhan University,Wuhan 430060,Hubei,China)

机构地区：[1]武汉大学人民医院麻醉科,湖北武汉430060 [2]武汉大学人民医院胸外科,湖北武汉430060

出　　处：《中国现代医生》2024年第8期20-24,共5页China Modern Doctor

基　　金：湖北省重点实验室开发项目(2021KFY052)。

摘　　要：目的旨在通过整合单细胞测序数据与TCGA数据库,探究在肺腺癌中泛素连接酶E2T(ubiquitin binding enzyme EZT,UBE2T)的作用,为揭示UBE2T在肺腺癌发生机制中的具体作用提供帮助。方法本研究从GEO数据库(GSE117570)下载了单细胞测序数据,并使用R语言进行数据质控和分析。同时,利用在线工具分析了TCGA数据库中与肺腺癌相关的数据。通过整合TCGA数据库与单细胞测序数据,分析UBE2T的潜在作用靶点。结果差异分析结果表明,UBE2T可作为肺腺癌独立的预后因子,并与患者预后不良相关。单细胞测序数据的整合分析显示,与UBE2T相关的10个基因主要分布在单核细胞和T细胞簇中。通过CancerSEA数据库的分析,发现UBE2T可能与细胞周期调控密切相关。结论UBE2T可能通过调控细胞周期来促进肺腺癌的发生和发展。Objective To investigate the role of ubiquitin binding enzyme EZT(UBE2T)in lung adenocarcinoma by integrating single-cell sequencing data with the TCGA database,in order to provide insights into the specific molecular mechanisms of UBE2T in lung adenocarcinoma.Methods Single-cell sequencing data was downloaded from the GEO database(GSE117570),and R language was used for quality control and analysis of the data.Additionally,online tools were employed to analyze lung adenocarcinoma-related data from the TCGA database.The potential target genes associated with UBE2T were identified by integrating TCGA and single-cell sequencing data.Results Differential analysis using the TCGA database successfully demonstrated that UBE2T could serve as an independent prognostic factor in lung adenocarcinoma,and it was correlated with poor patient outcomes.Integration of single-cell sequencing data revealed that UBE2T-associated genes were mainly distributed in mononuclear cells and T cells.Furthermore,analysis using the CancerSEA database suggested a close association between UBE2T and cell cycle regulation.Conclusion UBE2T may play a role in promoting the occurrence and development of lung adenocarcinoma through the regulation of the cell cycle.

关 键 词：肺腺癌 单细胞分析 TCGA数据库 

分 类 号：R734[医药卫生—肿瘤]